Workgroup of Biological and Medical Sciences


We are opened to a wide spectrum of collaboration with academic partners, partners from applied research and clinical partners. We can offer our help particularly in these fields:

- Histological and immunohistochemical analysis

- Genes and proteins analysis in tissues and cells

- Functional analysis of blood vessel contractility

- Testing of microbial and metabolic activity


- In vivo and in vitro research of TGF-β signaling in models of atherosclerosis and in endothelial cells

- In vitro antimicrobial activity testing methods based on internationally accepted standard methods, implementation and optimization of methodical approaches for screening of antimicrobial effect on biofilm-forming microorganisms

- Isolation, purifi cation and analysis of secreted/secerned proteins from microbial agents

Research Areas

- Research in the field of experimental atherogenesis. Study of the role of endoglin (CD105, TGF-βRIII) and its related signaling in atherogenesis

- Testing of antimicrobial activity, simulation of host-pathogen interaction via tissue explants

- Optimization of methodical procedures for drug-microbe interaction analysis

- Analysis and identification of proteins expressed/transported from microbes after interaction with host cells

- Research of the role of endoglin and its soluble form in pathogenesis of endothelial dysfunction and atherosclerosis

- Considering the role of soluble endoglin as a disease biomarker or possibly as an inducer of endothelial dysfunction in various cardiovascular diseases

- Finding of new candidate molecules with antimicrobial activity

- Study of molecular mechanisms of microbial pathogenesis

Main Capabilities

- Cultivation of endothelial cells (especially HUVECs)

- Breeding of various knockout hypercholesterolemic mice – models of experimental atherosclerosis

- Quantification of relevant proteins in cell cultures and sample tissues. Evaluation of tissue morphology. Assessment of mouse aorta function

- Cultivation of microbes under different conditions, determination of minimal inhibitory concentration of antimicrobial compounds, testing of microbial metabolic activity, detection and quantification of biofilm formation

- Microbial proteins isolation and purification

Key Research Equipment

- Histology and microscopy

- Light and fluorescent microscopy

- Western blot analysis

- Wire myograph technique

- Flow cytometry


- BSL2 microbiological labs

- Analytical devices: fluorescent microscope, confocal laser scanning microscope, spectrophotometers, microbiological incubators, autoclaves, biohazard safety boxes.

Partnerships & Collaborations

- prof. Carmelo Bernabeu, Center for Biological Research, Spanish National Research Council (CSIC), and Biomedical Research Networking Center on Rare Diseases, Madrid, Spain

- prof. Jose Lopez-Novoa, University of Salamanca, Spain

- prof. Stefan Chlopicki, Jagiellonian Centre for Experimental Therapeutics (JCET), Krakow, Poland

Main Projects

- 2015–2017 GACR project GA15-24015S: “Tissue and soluble endoglin and their importance in endothelial dysfunction and

atherogenesis in vivo and in vitro“

- 2015–2018 AZV project NV15-29225A: “Study of vaginal microbiota and its relationship to recurrent vulvovaginal discomfort”, principal investigator: Assoc. Prof. Vladimír Buchta.


- Publications in respected international journals with impact factor

- Presentations in domestic and international congresses

Are you interested in this expertise?

Please contact CPPT UK



Phone: +420 224 491 255

Experts and their department

Prof. PharmD. Petr Nachtigal, Ph.D.

Department of Biological and Medical Sciences


Poslední změna: 11. říjen 2019 18:41 
Sdílet na: Facebook Sdílet na: Twitter
Sdílet na:  
Máte dotaz?
Kontaktujte nás:

Centrum pro přenos poznatků a technologií, Univerzita Karlova



Tel.: +420 224 491 255

Adresa kanceláře:

Petrská 1180/3

110 00 Praha 1

Korespondenční adresa:

Ovocný trh 560/5

116 36 Praha 1

Jak k nám