Workgroup of Biological and Medical Sciences ****************************************************************************************** * ****************************************************************************************** ****************************************************************************************** * Offer ****************************************************************************************** We are opened to a wide spectrum of collaboration with academic partners, partners from ap and clinical partners. We can offer our help particularly in these fields: - Histological and immunohistochemical analysis - Genes and proteins analysis in tissues and cells - Functional analysis of blood vessel contractility - Testing of microbial and metabolic activity ****************************************************************************************** * Expertise ****************************************************************************************** - In vivo and in vitro research of TGF-? signaling in models of atherosclerosis and in end - In vitro antimicrobial activity testing methods based on internationally accepted standa implementation and optimization of methodical approaches for screening of antimicrobial ef forming microorganisms - Isolation, purifi cation and analysis of secreted/secerned proteins from microbial agent ****************************************************************************************** * Research Areas ****************************************************************************************** - Research in the field of experimental atherogenesis. Study of the role of endoglin (CD10 its related signaling in atherogenesis - Testing of antimicrobial activity, simulation of host-pathogen interaction via tissue ex - Optimization of methodical procedures for drug-microbe interaction analysis - Analysis and identification of proteins expressed/transported from microbes after intera cells - Research of the role of endoglin and its soluble form in pathogenesis of endothelial dys atherosclerosis - Considering the role of soluble endoglin as a disease biomarker or possibly as an induce dysfunction in various cardiovascular diseases - Finding of new candidate molecules with antimicrobial activity - Study of molecular mechanisms of microbial pathogenesis *========================================================================================= * Main Capabilities *========================================================================================= - Cultivation of endothelial cells (especially HUVECs) - Breeding of various knockout hypercholesterolemic mice – models of experimental atherosc - Quantification of relevant proteins in cell cultures and sample tissues. Evaluation of t Assessment of mouse aorta function - Cultivation of microbes under different conditions, determination of minimal inhibitory antimicrobial compounds, testing of microbial metabolic activity, detection and quantifica formation - Microbial proteins isolation and purification ****************************************************************************************** * Key Research Equipment ****************************************************************************************** - Histology and microscopy - Light and fluorescent microscopy - Western blot analysis - Wire myograph technique - Flow cytometry - ELISA, qRT-PCR - BSL2 microbiological labs - Analytical devices: fluorescent microscope, confocal laser scanning microscope, spectrop microbiological incubators, autoclaves, biohazard safety boxes. ****************************************************************************************** * Partnerships & Collaborations ****************************************************************************************** - prof. Carmelo Bernabeu, Center for Biological Research, Spanish National Research Counci Biomedical Research Networking Center on Rare Diseases, Madrid, Spain - prof. Jose Lopez-Novoa, University of Salamanca, Spain - prof. Stefan Chlopicki, Jagiellonian Centre for Experimental Therapeutics (JCET), Krakow *========================================================================================= * Main Projects *========================================================================================= - 2015–2017 GACR project GA15-24015S: “Tissue and soluble endoglin and their importance in dysfunction and atherogenesis in vivo and in vitro“ - 2015–2018 AZV project NV15-29225A: “Study of vaginal microbiota and its relationship to vulvovaginal discomfort”, principal investigator: Assoc. Prof. Vladimír Buchta. ****************************************************************************************** * Achievements ****************************************************************************************** - Publications in respected international journals with impact factor - Presentations in domestic and international congresses ****************************************************************************************** * Are you interested in this expertise? ****************************************************************************************** Please contact CPPT UK Web: www.cppt.cuni.cz/ [ URL "https://cppt.cuni.cz/"] Mail: transfer@cuni.cz Phone: +420 224 491 255 *========================================================================================= * Experts and their department *========================================================================================= Prof. PharmD. Petr Nachtigal, Ph.D. Department of Biological and Medical Sciences Web: www.portal.faf.cuni.cz/Profile/Nachtigal-Petr [ URL "http://www.portal.faf.cuni.cz/Pr Petr"]