Department of Histology and Embryology

# Histology

# Electron microscopy

# Immunohistochemistry

# Lungs, airways, vessels, cilia

Offer

- Our expertise in routine histology methods

- TEM research and diagnostics

- Immunohistochemistry and lectin histochemistry in paraffin and frozen sections

Know-how & Technologies

- Microscopic structure of lungs, respiratory passages, vessels, heart and placenta under experimental and pathological conditions, animal models

- Ultrastructure of respiratory cilia, high speed videomicroscopy, immunofluorescence

- Ultrastructural and molecular changes of cells and tissues

- Basic histology, conventional histochemistry, immunohistochemistry, lectin histochemistry

Laboratory Equipment

- Routine histological laboratory (tissue processor, microtomes, cryostat, vibratome)

- Special immunohistochemical laboratory

- Laboratory of electron microscopy (ultramicrotomes, sputter coater)

- Light and fluorescent microscopes

- Transmission electron microscope

- Computer image analyzers

Selected Publications

- Beránková, K., Uhlík, J., Hoňková, L., Pohunek, P.: Structural changes are present in bronchial mucosa of young children in risk for developing asthma. Pediatric Allergy and Immunology 25, 2, 2014: 136–142

- Uhlík, J., Šimůnková, P., Žaloudíková, M., Partlová, S., Jarkovský, J., Vajner, L.: Airway Wall Remodeling in Young and Adult Rats with Experimentally Provoked Bronchial Asthma. Int. Arch. Allergy Immunol. 164 (4), 2014: 289–300

- Hoňková, L., Uhlík, J., Beránková, K., Svobodová, T., Pohunek, P.: Epithelial basement membrane thickening is related to TGF-Beta 1 expression in children with chronic respiratory diseases. Pediatric Allergy and Immunology 25 (6), 2014: 593–599

- Novotný, T., Krejčí, J., Malíková. J., Švehlík, V., Wasserbauer, R., Uhlík, J, Vajner, L.: Mast-cell stabilization with sodium cromoglycate modulates pulmonary vessel wall remodeling during 4-day hypoxia in rats. Experimental Lung Research 41 (5), 2015: 283–292

- Djakow, J., Kramná, L., Dušátková, L., Uhlík, J., Pursiheimo, J.P., Svobodová, T., Pohunek, P., Cinek, O.: An effective combination of Sanger sequencing with next generation sequencing in the genetic diagnostics of primary ciliary dyskinesia. Pediatric Pulmonology 51 (5), 2016: 498–509

- Wilhelm, J., Vytášek, R., Uhlík, J., Vajner, L.: Oxidative stress in the developing rat brain due to production of reactive oxygen and nitrogen species. Histochemical and immunohistochemical study. Oxidative Medicine and Cellular Longevity, vol. 2016, Article ID 5057610, 12 pages, 2016. doi:10.1155/2016/5057610

- Novotný, T., Uhlík, J., Vajner, L.: Four-day pulse of sodium cromoglycate modulates pulmonary vessel wall remodeling during 21-day hypoxia in rats. Exp. Lung Res. 44 (1), 2018: 1–12