Offer

- Staining of tissue sections by common histological dyes

- Dissection of selected cells from tissue section and its transfer to microcaps followed by nucleic acids isolation

- Sterile dissection of alive cells from cell cultures for subsequent cultivation

- Creating of photo documentation of complete microdissection process for each sample

Expertise

- This laboratory is concerned with identifi cation and subsequent isolation of single cells from tissue sections and cell cultures

- Applied system allows work with fresh samples as well as with archive sections covered by medium

- Obtained samples serve for study of gene expression

- Additionally, used device enable to isolate concrete cell from heterogenic cell cultures and its subsequent cultivation

Members

- Assoc. Prof. Magdalena Chottová Dvořáková, M.D., Ph.D. – Research Group Leader

- Assoc. Prof. Jana Slavíková, M.D., Ph.D.

Selected Publications

- Chottova Dvorakova, M., Kruzliak, P., Rabkin, S.W. (2014) Role of neuropeptides in cardiomyopathies. Peptides 61, 1–6

- Mistrova, E., Wiegand, S., Sviglerova, J., Pfeil, U., Kuncova, J., Slavikova, J., Kummer, W., Chottova Dvorakova, M. (2014) Adrenomedullin and the calcitonin receptor-like receptor system mRNA expressions in the rat heart and sensory ganglia in experimentally-induced long-term diabetes. General Physiology and Biophysics 33 (2), 215–225

- Klenerova, V., Chottova Dvorakova, M., Skopek, P., Sida, P., Mistrova, E., Slavikova, J., Hynie, S. (2011) Expression of oxytocin receptor and its mRNA in two rat strains with different activity of HPA axis. Neuro Endocrinol Lett 32 (6), 805–810

- Chottova Dvorakova, M., Wiegand, S., Pesta, M., Slavikova, J., Reischig, J., Kuncova, J., Kummer, W. (2008) Expression of neuropeptide Y and its receptors Y1 and Y2 in the rat heart and its supplying autonomic and spinal sensory ganglia in experimentally induced diabetes. Neuroscience 151, 1016–1028

- Chottova Dvorakova, M., Pfeil, U., Kuncová, J., Švíglerová, J., Galvis, G., Krasteva, G., König, P., Grau, V., Slavíková, J., Kummer, W. (2006) Down-regulation of vasoactive intestinal peptide and altered expression of its receptors in rat diabetic cardiomyopathy. Cell Tissue Res 323, 383–393

Are you interested in this expertise?

Please contact CPPT UK

Web: www.cppt.cuni.cz/

Mail: transfer@cuni.cz

Phone: +420 224 491 255

Experts and their department

Assoc. Prof. Magdalena Chottová Dvořáková, M.D., Ph.D.

Institute of Physiology

Web: www.biomedic-plzen.cz