Laboratory of LASER Microdissection












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            * Offer
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            - Staining of tissue sections by common histological dyes

            - Dissection of selected cells from tissue section and its transfer to microcaps followed 
            isolation

            - Sterile dissection of alive cells from cell cultures for subsequent cultivation
            - Creating of photo documentation of complete microdissection process for each sample






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            * Expertise
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            - This laboratory is concerned with identifi cation and subsequent isolation of single cel
            sections and cell cultures
            - Applied system allows work with fresh samples as well as with archive sections covered b

            - Obtained samples serve for study of gene expression
            - Additionally, used device enable to isolate concrete cell from heterogenic cell cultures
            subsequent cultivation






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            * Members

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            - Assoc. Prof. Magdalena Chottová Dvořáková, M.D., Ph.D. – Research Group Leader
            - Assoc. Prof. Jana Slavíková, M.D., Ph.D.






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            * Selected Publications

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            - Chottova Dvorakova, M., Kruzliak, P., Rabkin, S.W. (2014) Role of neuropeptides in cardi
            Peptides 61, 1–6

            - Mistrova, E., Wiegand, S., Sviglerova, J., Pfeil, U., Kuncova, J., Slavikova, J., Kummer
            Dvorakova, M. (2014) Adrenomedullin and the calcitonin receptor-like receptor system mRNA 
            the rat heart and sensory ganglia in experimentally-induced long-term diabetes. General Ph

            Biophysics 33 (2), 215–225
            - Klenerova, V., Chottova Dvorakova, M., Skopek, P., Sida, P., Mistrova, E., Slavikova, J.
            (2011) Expression of oxytocin receptor and its mRNA in two rat strains with different acti

            Neuro Endocrinol Lett 32 (6), 805–810
            - Chottova Dvorakova, M., Wiegand, S., Pesta, M., Slavikova, J., Reischig, J., Kuncova, J.
            W. (2008) Expression of neuropeptide Y and its receptors Y1 and Y2 in the rat heart and it

            autonomic and spinal sensory ganglia in experimentally induced diabetes. Neuroscience 151,
            - Chottova Dvorakova, M., Pfeil, U., Kuncová, J., Švíglerová, J., Galvis, G., Krasteva, G.
            Grau, V., Slavíková, J., Kummer, W. (2006) Down-regulation of vasoactive intestinal peptid

            expression of its receptors in rat diabetic cardiomyopathy. Cell Tissue Res 323, 383–393





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            * Are you interested in this expertise?
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            Please contact CPPT UK

            Web: www.cppt.cuni.cz/ [ URL "https://cppt.cuni.cz/"]
            Mail: transfer@cuni.cz
            Phone: +420 224 491 255






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            * Experts and their department

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            Assoc. Prof. Magdalena Chottová Dvořáková, M.D., Ph.D.
            Institute of Physiology

            Web: www.biomedic-plzen.cz [ URL "http://www.biomedic-plzen.cz"]