Department of Histology and Embryology












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            *
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            # Histology

            # Electron microscopy
            # Immunohistochemistry
            # Lungs, airways, vessels, cilia






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            * Offer
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            - Our expertise in routine histology methods

            - TEM research and diagnostics
            - Immunohistochemistry and lectin histochemistry in paraffin and frozen sections






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            * Know-how & Technologies
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            - Microscopic structure of lungs, respiratory passages, vessels, heart and placenta under 
            pathological conditions, animal models
            - Ultrastructure of respiratory cilia, high speed videomicroscopy, immunofluorescence

            - Ultrastructural and molecular changes of cells and tissues
            - Basic histology, conventional histochemistry, immunohistochemistry, lectin histochemistr






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            * Laboratory Equipment
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            - Routine histological laboratory (tissue processor, microtomes, cryostat, vibratome)
            - Special immunohistochemical laboratory
            - Laboratory of electron microscopy (ultramicrotomes, sputter coater)

            - Light and fluorescent microscopes
            - Transmission electron microscope
            - Computer image analyzers






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            * Selected Publications

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            - Beránková, K., Uhlík, J., Hoňková, L., Pohunek, P.: Structural changes are present in br
            young children in risk for developing asthma. Pediatric Allergy and Immunology 25, 2, 2014

            - Uhlík, J., Šimůnková, P., Žaloudíková, M., Partlová, S., Jarkovský, J., Vajner, L.: Airw
            Remodeling in Young and Adult Rats with Experimentally Provoked Bronchial Asthma. Int. Arc
            Immunol. 164 (4), 2014: 289–300

            - Hoňková, L., Uhlík, J., Beránková, K., Svobodová, T., Pohunek, P.: Epithelial basement m
            thickening is related to TGF-Beta 1 expression in children with chronic respiratory diseas
            Allergy and Immunology 25 (6), 2014: 593–599

            - Novotný, T., Krejčí, J., Malíková. J., Švehlík, V., Wasserbauer, R., Uhlík, J, Vajner, L
            stabilization with sodium cromoglycate modulates pulmonary vessel wall remodeling during 4
            rats. Experimental Lung Research 41 (5), 2015: 283–292

            - Djakow, J., Kramná, L., Dušátková, L., Uhlík, J., Pursiheimo, J.P., Svobodová, T., Pohun
            Cinek, O.: An effective combination of Sanger sequencing with next generation sequencing i
            diagnostics of primary ciliary dyskinesia. Pediatric Pulmonology 51 (5), 2016: 498–509

            - Wilhelm, J., Vytášek, R., Uhlík, J., Vajner, L.: Oxidative stress in the developing rat 
            production of reactive oxygen and nitrogen species. Histochemical and immunohistochemical 
            Medicine and Cellular Longevity, vol. 2016, Article ID 5057610, 12 pages, 2016. doi:10.115

            - Novotný, T., Uhlík, J., Vajner, L.: Four-day pulse of sodium cromoglycate modulates pulm
            remodeling during 21-day hypoxia in rats. Exp. Lung Res. 44 (1), 2018: 1–12






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            * Team Members
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            - Doc. MVDr. Luděk Vajner, CSc.
            - MUDr. Petra Dvořáková
            - MUDr. Jiří Uhlík, Ph.D.






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            * Are you interested in this expertise?

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            Please contact CPPT UK
            Web: www.cppt.cuni.cz/ [ URL "https://cppt.cuni.cz/"]

            Mail: transfer@cuni.cz
            Phone: +420 224 491 255






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            * Experts and their department
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            Jiří Uhlík, M.D., Ph.D.
            Department of Histology and Embryology
            Web: http://histologie.lf2.cuni.cz/ [ URL "http://histologie.lf2.cuni.cz/"]






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            * Klíčová slova

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            # Histologie
            # Elektronová mikroskopie

            # Imunohistochemie
            # Plíce, dýchací cesty, cévy, řasinky