Laboratory of Preclinical Studies


- Model of healing of cutaneous wounds in non-diabetic and diabetic rats (both DMI and DMII)

- Porcine model of healing of normal cutaneous wounds

- Porcine model of healing of full-thickness cutaneous wounds experimentally infected with bacterial biofilm

- Porcine model of osteochondral deffect in knee joint

- Porcine models of fractures of femur

- In vivo testing of materials for stomatology and maxillofacial surgery

- In vivo testing of materials for soft tissue augmenations

- In vivo testing of wide spectrum of implants and prostheses

- Murine models of experimental infection of Cryptosporidium (both for basic research and evaluation of antiprotozoal drugs)


The Animal Research Facility is designed to provide all experiments on following animals:

- mice

- rats

- hamsters

- gerbils

- guinea-pigs

- rabbits

- pigs

- sheeps

- goats.

In addition, there are established animal models allowing to do contract research and scientifi c cooperation, especially in regenerative medicine and experimental surgery.

Research Area & Excellence

The section for small animals allows housing in conventional conditions and in the barrier. This experimental facilities includes fully equipped laboratory allowing to perform all the in vivo experiments, including surgical procedures.

The section for the large animals includes clean rooms, in which there are two fully equipped operating theatres and experimental ICU.


- Pavel Klein, Ph.D., M.Sc. – Research Group Leader

- Ilona Lacinová

- Markéta Navrátilová

- Ivana Ocskaiová

- Kristýna Šmídová

Selected Publications

- Bobula T., Běťák J., Buffa R., Moravcová M., Klein P., Židek O., Chadimová V., Pospíšil R., Velebný V.: Solidstate photocrosslinking of hyaluronan microfibres, Carbohydrate Polymers 2015, 125: 153–60

- Kučera L., Wiunfurterová R., Dvořáková J., Kučera J., Pravda M., Foglarová M., Švík K., Klein P., Velebný V.: Chondrocyte Cultivation in Hyaluronan-Tyramine Cross-Linked Hydrogel. International Journal of Polymeric Materials 2015, 64 (13): 661–74

- Kučera J., Sojka, M., Pavlík, V., Szuszkiewicz, K., Velebný, V., Klein, P.: Multispecies biofilm in an artificial wound bed – a novel model for in vitro assessment of solid antimicrobial dressings. Journal of Microbiological Methods 2014, 103: 18 24

- Klein, P., Kleinová, T., Volek, Z., Šimůnek, J.: Effect of Cryptosporidium parvum infection on the absorptive capacity and paracellular permeability of the small intestine in neonatal calves. Veterinary Parasitology 2008, 152 (1–2): 53–9

- Klein, P.: Preventive and therapeutic efficacy of halofuginone-lactate againstCryptosporidium parvum in spontaneously infected calves: A centralised, randomised, double-blind, placebo-controlled study. The Veterinary Journal 2008, 177 (3): 429–31

- Klein, P., Cirioni, O., Giacometti, A., Scalise, G.: In vitro and in vivo activity of aurintricarboxylic acid preparations against Cryptosporidium parvum. Journal of Antimicrobial Chemotherapy 2008, 62 (5): 1101–4

- Widmer, G., Klein, P., Bonilla, R.: Adaptation of Cryptosporidium oocysts to different excystation conditions. Parasitology 2007, 134: 1583–8

- Klein, P., Moravcová, J., Kleinová, T., Volek, Z., Skřivanová, V.: Assessment of intestinal permeability in preruminant calves by lactulose/mannitol test. Journal of Animal and Feed Sciences 2007; 16 (1): 43–52

- Klein, P.: Preparation of the formaurindicarboxylic acid base and its derivations and use. US 8030353 B2

- Bobula, T., Pospíšil, R., Buffa, R., Růžičková, J., Moravcová, M., Klein, P., Velebný, V.: Photoreactive derivative of hyaluronic acid, method of preparation thereof, 3d-crosslinked derivative of hyaluronic acid, method of preparation and use thereof. WO 2014082608 A1

- Šmejkalová, D., Huerta-Angeles, G., Bobek, M., Hermannová, M., Vištejnová, L., Novotný, J., Příkopová, E., Nešporová, K., Němcová, M., Šlezingerová, K., Kulhánek,J., Čožíková, D., Šógorková, J., Kučera, J., Klein, P., Velebný, V.: C6-c18-acylated derivative of hyaluronic acid, method of preparation thereof, nanomicellar composition on its basis, method of preparation thereof and method of preparation stabilized nanomicellar composition, and use thereof. WO 2014082609 A1

- Ščudlová, J., Běťák, J., Wolfová, L., Buffa, R., Šlezingrová, K., Klein, P., Matějková, I., Bobek, M., Pitucha, T., Velebný, V., Šuláková, R.: Fibres based on hydrophobized derivatives of hyaluronan, method of their preparation and use, textiles on base thereof and use thereof. WO2014082611 A8

- Běťák, J., Buffa, R., Němcová, M., Pitucha, T., Kulhánek, J., Matějková, I., Nováková, J., Vištejnová, L., Klein, P., Kubíčková, G., Broulíková, M., Felgrová, M., Velebný, V.: Fibres sans fin à base d‘hyaluronane sélectivement oxydé en position 6 du groupe n-acétyl-d-glucosamine, leur préparation et utilisation, filés, fibres courtes, fils et tissus constitués de celles-ci et procédé pour la modification de celles-ci. WO 2014082610 A1

Are you interested in this expertise?

Please contact CPPT UK



Phone: +420 224 491 255

Experts and their Department

Pavel Klein, Ph.d., M.Sc.

Biomedical Center


Poslední změna: 11. říjen 2019 18:41 
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