Laboratory of Preclinical Studies ****************************************************************************************** * ****************************************************************************************** ****************************************************************************************** * Offer ****************************************************************************************** - Model of healing of cutaneous wounds in non-diabetic and diabetic rats (both DMI and DMI - Porcine model of healing of normal cutaneous wounds - Porcine model of healing of full-thickness cutaneous wounds experimentally infected with biofilm - Porcine model of osteochondral deffect in knee joint - Porcine models of fractures of femur - In vivo testing of materials for stomatology and maxillofacial surgery - In vivo testing of materials for soft tissue augmenations - In vivo testing of wide spectrum of implants and prostheses - Murine models of experimental infection of Cryptosporidium (both for basic research and antiprotozoal drugs) ****************************************************************************************** * Expertise ****************************************************************************************** The Animal Research Facility is designed to provide all experiments on following animals: - mice - rats - hamsters - gerbils - guinea-pigs - rabbits - pigs - sheeps - goats. In addition, there are established animal models allowing to do contract research and scie cooperation, especially in regenerative medicine and experimental surgery. ****************************************************************************************** * Research Area & Excellence ****************************************************************************************** The section for small animals allows housing in conventional conditions and in the barrier experimental facilities includes fully equipped laboratory allowing to perform all the in including surgical procedures. The section for the large animals includes clean rooms, in which there are two fully equip theatres and experimental ICU. ****************************************************************************************** * Members ****************************************************************************************** - Pavel Klein, Ph.D., M.Sc. – Research Group Leader - Ilona Lacinová - Markéta Navrátilová - Ivana Ocskaiová - Kristýna Šmídová ****************************************************************************************** * Selected Publications ****************************************************************************************** - Bobula T., Běťák J., Buffa R., Moravcová M., Klein P., Židek O., Chadimová V., Pospíšil Solidstate photocrosslinking of hyaluronan microfibres, Carbohydrate Polymers 2015, 125: 1 - Kučera L., Wiunfurterová R., Dvořáková J., Kučera J., Pravda M., Foglarová M., Švík K., Velebný V.: Chondrocyte Cultivation in Hyaluronan-Tyramine Cross-Linked Hydrogel. Internat Polymeric Materials 2015, 64 (13): 661–74 - Kučera J., Sojka, M., Pavlík, V., Szuszkiewicz, K., Velebný, V., Klein, P.: Multispecies artificial wound bed – a novel model for in vitro assessment of solid antimicrobial dressi Microbiological Methods 2014, 103: 18 24 - Klein, P., Kleinová, T., Volek, Z., Šimůnek, J.: Effect of Cryptosporidium parvum infect absorptive capacity and paracellular permeability of the small intestine in neonatal calve Parasitology 2008, 152 (1–2): 53–9 - Klein, P.: Preventive and therapeutic efficacy of halofuginone-lactate againstCryptospor in spontaneously infected calves: A centralised, randomised, double-blind, placebo-control Veterinary Journal 2008, 177 (3): 429–31 - Klein, P., Cirioni, O., Giacometti, A., Scalise, G.: In vitro and in vivo activity of au acid preparations against Cryptosporidium parvum. Journal of Antimicrobial Chemotherapy 20 4 - Widmer, G., Klein, P., Bonilla, R.: Adaptation of Cryptosporidium oocysts to different e conditions. Parasitology 2007, 134: 1583–8 - Klein, P., Moravcová, J., Kleinová, T., Volek, Z., Skřivanová, V.: Assessment of intesti in preruminant calves by lactulose/mannitol test. Journal of Animal and Feed Sciences 2007 - Klein, P.: Preparation of the formaurindicarboxylic acid base and its derivations and us - Bobula, T., Pospíšil, R., Buffa, R., Růžičková, J., Moravcová, M., Klein, P., Velebný, V derivative of hyaluronic acid, method of preparation thereof, 3d-crosslinked derivative of method of preparation and use thereof. WO 2014082608 A1 - Šmejkalová, D., Huerta-Angeles, G., Bobek, M., Hermannová, M., Vištejnová, L., Novotný, E., Nešporová, K., Němcová, M., Šlezingerová, K., Kulhánek,J., Čožíková, D., Šógorková, J. Klein, P., Velebný, V.: C6-c18-acylated derivative of hyaluronic acid, method of preparati nanomicellar composition on its basis, method of preparation thereof and method of prepara nanomicellar composition, and use thereof. WO 2014082609 A1 - Ščudlová, J., Běťák, J., Wolfová, L., Buffa, R., Šlezingrová, K., Klein, P., Matějková, Pitucha, T., Velebný, V., Šuláková, R.: Fibres based on hydrophobized derivatives of hyalu their preparation and use, textiles on base thereof and use thereof. WO2014082611 A8 - Běťák, J., Buffa, R., Němcová, M., Pitucha, T., Kulhánek, J., Matějková, I., Nováková, J L., Klein, P., Kubíčková, G., Broulíková, M., Felgrová, M., Velebný, V.: Fibres sans fin ? d‘hyaluronane sélectivement oxydé en position 6 du groupe n-acétyl-d-glucosamine, leur pré utilisation, filés, fibres courtes, fils et tissus constitués de celles-ci et procédé pour de celles-ci. WO 2014082610 A1 ****************************************************************************************** * Are you interested in this expertise? ****************************************************************************************** Please contact CPPT UK Web: www.cppt.cuni.cz/ [ URL "https://cppt.cuni.cz/"] Mail: transfer@cuni.cz Phone: +420 224 491 255 ****************************************************************************************** * Experts and their Department ****************************************************************************************** Pavel Klein, Ph.d., M.Sc. Biomedical Center Web: http://www.biomedic-plzen.cz/cz [ URL "http://www.biomedic-plzen.cz/cz"]