Offer

- Preparation of biological samples prior to proteomic analysis, i.e. homogenisation, isolation, purification and fractionation

- One-dimensional polyacrylamide gel electrophoresis

- Two-dimensional polyacrylamide gel electrophoresis

- Differential polyacrylamide gel electrophoresis (DIGE)

- Gel imaging in visible and UV spectrum

- Gel image analysis including statistical evaluation

- Protein identification by tandem mass spektrometry

- Biofilm analysis of implantable devices and extracorporeal circuits

Expertise

The research team focuses on analysis of complex protein mixtures, including

- human and animal tissues and body fluids,

- as well as their derivatives, using a proteomic technology.

This center major advantage lies in its experience with processing and identification of real clinical samples, particularly

- uremic blood plasma,

- dialysate/ultrafiltrate of uremic blood plasma and

- dialysis membrane biofilm.

Members

- Jan Mareš, M.D., Ph.D. – Research Group Leader

- Jiří Moravec, Ph.D., M.Sc.

- Zdeněk Tůma, Ph.D., M.Sc.

- Jelena Kaňáková

- Ludmila Reifová

Selected Publications

- Mares J, Thongboonkerd V, Tuma Z, Moravec J, Matejovic M: Specific adsorption of some complement activation proteins to polysulfone dialysis membranes during hemodialysis. Kidney Int 2009

- Mares J, Richtrova P, Hricinova A, Tuma Z, Moravec J, Lysak D, Matejovic M: Proteomic profiling of blood-dialyzer interactome reveals involvement of lectin complement pathway in hemodialysis- induced inflammatory response. Proteomics - Clinical Applications 2010, 4: 829–838

- Mares J, Thongboonkerd V, Tuma Z, Moravec J, Karvunidis T, Matejovic M: Proteomic Analysis of Proteins Bound to Adsorption Units of Extracorporeal Liver Support Systém under Clinical Conditions. J Proteome Res 2009, 8: 1756–1764

- Thongboonkerd V, Chiangjong W, Mares J, Moravec J, Tuma Z, Karvunidis T, Sinchaikul S, Chen ST, Opatrny K, Matejovic M: Altered plasma proteome during an early phase of peritonitis-induced sepsis. Clin Sci (Lond) 2009, 116: 721–730

- Molacek J, Mares J, Treska V, Houdek K, Baxa J: Proteomic analysis of the abdominal aortic aneurysm wall. Surg Today 2014, 44: 142–151

Are you interested in this expertise?

Please contact CPPT UK

Web: www.cppt.cuni.cz/

Mail: transfer@cuni.cz

Phone: +420 224 491 255

Experts and their department

Jan Mareš, M.D., Ph.D.

Biomedical Center

Web: www.biomedic-plzen.cz