Laboratory of Proteomics












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            * Offer
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            - Preparation of biological samples prior to proteomic analysis, i.e. homogenisation, isol

            purification and fractionation
            - One-dimensional polyacrylamide gel electrophoresis

            - Two-dimensional polyacrylamide gel electrophoresis
            - Differential polyacrylamide gel electrophoresis (DIGE)
            - Gel imaging in visible and UV spectrum

            - Gel image analysis including statistical evaluation
            - Protein identification by tandem mass spektrometry
            - Biofilm analysis of implantable devices and extracorporeal circuits






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            * Expertise

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            The research team focuses on analysis of complex protein mixtures, including
            - human and animal tissues and body fluids,

            - as well as their derivatives, using a proteomic technology.

            This center major advantage lies in its experience with processing and identification of r

            samples, particularly
            - uremic blood plasma,
            - dialysate/ultrafiltrate of uremic blood plasma and

            - dialysis membrane biofilm.





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            * Members
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            - Jan Mareš, M.D., Ph.D. – Research Group Leader

            - Jiří Moravec, Ph.D., M.Sc.
            - Zdeněk Tůma, Ph.D., M.Sc.
            - Jelena Kaňáková

            - Ludmila Reifová





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            * Selected Publications
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            - Mares J, Thongboonkerd V, Tuma Z, Moravec J, Matejovic M: Specific adsorption of some co

            activation proteins to polysulfone dialysis membranes during hemodialysis. Kidney Int 2009
            - Mares J, Richtrova P, Hricinova A, Tuma Z, Moravec J, Lysak D, Matejovic M: Proteomic pr
            dialyzer interactome reveals involvement of lectin complement pathway in hemodialysis- ind

            response. Proteomics - Clinical Applications 2010, 4: 829–838
            - Mares J, Thongboonkerd V, Tuma Z, Moravec J, Karvunidis T, Matejovic M: Proteomic Analys
            Bound to Adsorption Units of Extracorporeal Liver Support Systém under Clinical Conditions

            2009, 8: 1756–1764
            - Thongboonkerd V, Chiangjong W, Mares J, Moravec J, Tuma Z, Karvunidis T, Sinchaikul S, C
            K, Matejovic M: Altered plasma proteome during an early phase of peritonitis-induced sepsi

            (Lond) 2009, 116: 721–730
            - Molacek J, Mares J, Treska V, Houdek K, Baxa J: Proteomic analysis of the abdominal aort
            Surg Today 2014, 44: 142–151






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            * Are you interested in this expertise?

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            Please contact CPPT UK
            Web: www.cppt.cuni.cz/ [ URL "https://cppt.cuni.cz/"]

            Mail: transfer@cuni.cz
            Phone: +420 224 491 255






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            * Experts and their department
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            Jan Mareš, M.D., Ph.D.
            Biomedical Center
            Web: www.biomedic-plzen.cz [ URL "http:// www.biomedic-plzen.cz"]