Laboratory of Cystic Fibrosis Pathogenesis ****************************************************************************************** * ****************************************************************************************** "Our goal is the development of an effective antibody prophylaxis of CF patients preventin infections caused by P. aeruginosa and B. cepacia complex as well as the restoration of CF administration of a stable mRNA. “ ****************************************************************************************** * Offer ****************************************************************************************** We can provide basic services in a general field of an antigen selection and immunogen pre - Pseudomonas aeruginosa adherence assay using fluorescence labeled bacteria and lung epit - The effect of prophylactic IgY against virulence factors of P. aeruginosa and complex ca - We also provide molecular techniques, such as mRNA synthesis or cell transfection - Currently, the mouse CF lung model is being developed ****************************************************************************************** * Know-how & Technologies ****************************************************************************************** - Antigen selection and immunogen preparation: computer design of peptide antigens, peptid carrier, purification of egg yolk antibody and its affinity purification (using our patent - Animal treatment: aerosol inhalation (PARI nebulizer), intratracheal instillation (mouse - Cell culture handling: lung epithelium cell cultures (CuFi and NuLi) cultivation, cell t - Developed assay: bacterial adherence assay using fluorescence labeled bacteria and lung – this procedure is currently employed in the development of antibacterial antibody prophy - Assays we used: ELISA, Western blotting, immunostaining with fluorescence microscopy, mi efflux assay, luminescence determination (Tecan Infinite M200 Pro) - Design of stable mRNA and its synthesis ****************************************************************************************** * Content of Research ****************************************************************************************** - Administration of egg yolk antibody against virulence factors of bacteria as an excellen prophylaxis of microbial infections that can prevent damage of lung epithelium of cystic f - Transfection of epithelial cells with synthetized stable CFTR mRNA that could restore CF The restoration of CF airway function is tested using a chloride ion channel activity dete fluorescent microplate assay. ****************************************************************************************** * Key Research Equipment ****************************************************************************************** - Spectrofluorometer (Tecan Infinite M200 Pro) - Ultracentrifuge Optima XPN (Beckman Coulter) - ELISA reader Sunrise (Tecan) - Hydro Flex ELISA (Tecan) - Microscop Nikon ECLIPSE TE2002-U (with IS-Elements AR 2.30 software) ****************************************************************************************** * Partnerships & Collaborations ****************************************************************************************** *========================================================================================= * Ademic Partners *========================================================================================= Department of Pathology, 3rd Faculty of Medicine, Charles University | Department of Medic 2nd Faculty of Medicine, Charles University and University Hospital Motol | Department of Faculty of Science, Masaryk University *========================================================================================= * Private and Public Sector *========================================================================================= National Institute of Public Health in Prague | Institute for Clinical and Experimental Me (IKEM),Prague | Center for Cystic Fibrosis Patients, z.s ****************************************************************************************** * Main Projects ****************************************************************************************** Coordinator of grants from the Grant Agency of Czech Republic: Centre of drug-dietary supp interactions and nutrigenetics (GA CR P303/12/G163) and Grant Agency of Charles University collaborator of 16 grant projects from GA CR, 4 grants of the Grant Agency for Development and 3 grants GA UK ****************************************************************************************** * Achievements ****************************************************************************************** - HODEK, P., TREFIL, P., ŠIMŮNEK, J., HUDEČEK, J., STIBOROVÁ, M.; Optimized protocol of ch (IgY) purification providing electrophoretically homogenous preparations. Int. J. Electroc 113–124 (2013) - HADRABOVÁ, J., MAJEROVÁ, B., ČERNÁ, V., MOSEROVÁ, M., HOLUŠA, R., MANDYS, V., STIBOROVÁ, HODEK, P.; Chicken immunoglobulins for prophylaxis: Effect of inhaled antibodies on inflam in rat airways. J. Appl. Biomed., 13, 19–22 (2015) - NOSKOVÁ, L., KUBÍČKOVÁ, B., VAŠKOVÁ, L., BLÁHOVÁ, B., WIMMEROVÁ, M., STIBOROVÁ, M., HODE Fluorescent cellular assay for screening agents inhibiting Pseudomonas aeruginosa adherenc 1945–1953 (2015) - VAŠKOVÁ, L., NOSKOVÁ, L., BLÁHOVÁ, B., WIMMEROVÁ, M., DŘEVÍNEK, P., KUBÍČKOVÁ, B., STIBO HODEK, P.; Evaluation of anti-PAIIL lectin hen yolk antibody as an agent inhibiting Pseudo adherence to epithelial cells. Monatsh. Chem.147, 889–896 (2016) - KUBÍČKOVÁ, B., HADRABOVÁ, J., VAŠKOVÁ, L., MANDYS, V., STIBOROVÁ, M., HODEK, P.: Suscept airways to Pseudomonas aeruginosa infection: mouse neuraminidase model; Monatsh. Chem., 14 (2017) ****************************************************************************************** * Are you interested in this expertisa? ****************************************************************************************** Please contact CPPT UK Web: www.cppt.cuni.cz/ [ URL "https://cppt.cuni.cz/"] Mail: transfer@cuni.cz Tel.: +420 224 491 255 ****************************************************************************************** * Experts and their department ****************************************************************************************** Prof. RNDr. Petr Hodek, CSc. Department of Biochemistry Web: https://twitter.com/konvalinka_lab [ URL "https://twitter.com/konvalinka_lab"]