Laboratory of Tumor Biology












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            * Offer
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            - Nucleic acid isolation from various cell types, frozen tissues and FFPE tissue samples

            - Quality control of nucleic acids
            - Analysis of relative gene expression and comparison of expression profiles of your sampl

            - Preparation of gene expression microarry experiments
            - Detection of single-nucleotide polymorphisms and mutations
            - Fluorescence microscopy of cell cultures and tissue samples

            - Statistical analysis of results
            - Biological and functional interpretation of your results
            - Consultation of experimental design and selection of appropriate methodology for given p

            - Layout of project including time and cost estimation





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            * Expertise
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            - Main goal of the Laboratory of Tumour Biology is to study novel prognostic and predictiv

            the capacity to improve treatment of oncology patients.
            - We use colorectal cancer as a tumour model, because it is one of the most common maligna
            worldwide as well as in the Czech republic.

            - Within individual projects we measure expression levels of selected genes during tumour 
            assess markers of cancer stem cells in the tumour samples or detect and characterize circu
            cells in the peripheral blood, including whole genome analysis of individual cells.






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            * Members

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            - Pavel Pitule, Ph.D., M.Sc. – Research Group Leader
            - Pavel Ostašov, Ph.D., M.Sc.

            - Dipl.-Biol. Jana-Alleta Thiele
            - Eva Královcová
            - Petr Hošek, M.Sc.

            - Assoc. Prof. Martin Pešta, Ph.D., M.Sc.
            - Ludmila Vodičková, M.D., Ph.D.
            - Pavel Vodička, M.D., Ph.D.

            - Jiří Polívka, M.Sc.
            - Dr. James Hicks






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            * Selected Publications
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            - Pitule P, Vycital O, Bruha J, Novak P, Hosek P, Treska V, Hlavata I, Soucek P, Kralickov
            Differential expression and prognostic role of selected genes in colorectal cancer patient
            Res. 2013 Nov; 33 (11): 4855–65

            - Pitule P, Cedikova M, Daum O, Vojtisek J, Vycital O, Hosek P, Treska V, Hes O, Kralickov
            Immunohistochemical detection of cancer stem cell related markers CD44 and CD133 in metast
            cancer patients. Biomed Res Int. 2014; 2014: 432139

            - Pitule P., Cedikova M., Treska V., Kralickova M., Liska V.: Assessing colorectal cancer 
            one step closer to tailored medicine. Journal of Applied Biomedicine. 2013 11 (3): 115–129
            - Liska V, Vycital O, Daum O, Novak P, Treska V, Bruha J, Pitule P, Holubec L.: Infiltrati

            carcinoma by S100+ dendritic cells and CD57+ lymphocytes as independent prognostic factors
            surgical treatment. Anticancer Res. 2012 May; 32 (5): 2129–32
            - Hlavata I, Mohelnikova-Duchonova B, Vaclavikova R, Liska V, Pitule P, Novak P, Bruha J, 

            Holubec L, Treska V, Vodicka P, Soucek P.: The role of ABC transporters in progression and
            of colorectal cancer. Mutagenesis. 2012 Mar; 27 (2): 187–96
            - Slyskova J, Cordero F, Pardini B, Korenkova V, Vymetalkova V, Bielik L, Vodickova L, Pit

            Matejka VM, Levy M, Buchler T, Kubista M, Naccarati A, Vodicka P.: Post-treatment recovery
            DNA repair capacity and gene expression levels in colorectal cancer patients. Mol Carcinog
            doi: 10.1002/mc.22141






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            * Are you interested in this expertise?

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            Please contact CPPT UK
            Web: www.cppt.cuni.cz/ [ URL "https://cppt.cuni.cz/"]

            Mail: transfer@cuni.cz
            Phone: +420 224 491 255






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            * Experts and their Department
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            Pavel Pitule, Ph.D., M.Sc.
            Biomedical Center
            Web: http://www.biomedic-plzen.cz/cz [ URL "http://www.biomedic-plzen.cz/cz"]