Laboratory of Reproductive Medicine












            ******************************************************************************************

            *
            ******************************************************************************************




            ******************************************************************************************

            * Offer
            ******************************************************************************************
            - In vitro oocyte maturation (IVM), in vitro fertilization (IVF) and embryo culture (IVC)

            - Flow cytometry and sorting of sperm (FACSAria, FACSVerse, BD Bioscience)
            - Intracytoplasmic sperm injection (ICSI)

            - Immunocytochemistry of oocytes and embryo
            - Epi-fluorescent and confocal laser-scanning microscopy, live cell imaging (Olympus, IX83
            - Western Blotting (Biorad)






            ******************************************************************************************
            * Expertise

            ******************************************************************************************
            Laboratory of Reproductive medicine is focused on:


            - spermatogenesis,
            - oocyte maturation,
            - mechanisms of fertilization,

            - pre-implanted embryonic development in vitro.

            In addition to in vitro experiments, mouse model offers in vivo testing of selected enviro

            polutants.





            ******************************************************************************************

            * Members
            ******************************************************************************************
            - Jan Nevoral, Ph.D., M.Sc.

            - M.V.D. Olga García Álvarez, Ph.D.
            - Miriam Štiavnická, M.Sc.
            - Hedvika Řimnáčová, M.Sc.

            - Tereza Fenclová, M.Sc.
            - Václav Rúčka, M.Sc.
            - Prof. Milena Králíčková, M.D., Ph.D.






            ******************************************************************************************
            * Selected Publications

            ******************************************************************************************
            - del Barco-Trillo J, García-Álvarez O, Soler AJ, Tourmente M et al.: A cost for high leve
            competition in rodents: increased sperm DNA fragmentation. Proceedings B. 2016. DOI:10.108

            - García-Álvarez O, Maroto-Morales A, Jiménez-Rabadán P, Ramón M et al.: Effect of differe
            additives on capacitation of frozen-thawed ram spermatozoa as a potential replacement for 
            serum. Theriogenology 2015, 84: 948–955. DOI:10.1016/j.theriogenology.2015.05.032

            - García-Álvarez O, Maroto-Morales A, Ramón M, del Olmo E et al.: Dynamic of sperm subpopu
            motility and membrane status in thawed ram spermatozoa incubated under conditions that sup
            capacitation and fertilization. Reproduction, Fertility and Development 2014, 26: 725–732.

            RD13034
            - Nevoral J, Petr j, Gelaude A, Bodart JF et al.: Dual Effects of Hydrogen Sulfide Donor o
            Cumulus Expansion of Porcine Cumulus-Oocyte Complexes. PLOS ONE 07/2014; 9 (7): e99613. DO

            journal.pone.0099613
            - Nevoral J, Sutovsky P: Involvement of epigenetic changes and ubiquitin-dependent proteol
            pronuclear development of mammalian zygote. In: Heide Schatten (ed.): Animal Models and Hu

            New York: John Wiley & Sons, 2016. In Press
            - Nevoral J, Zalmanova T, Bodart JF, Petr J et al.: Endogenously produced hydrogen sulfide
            porcine oocyte maturation in vitro. Nitric Oxide: Biology and Chemistry 12/2015, 51 (12). 

            j.niox.2015.09.007





            ******************************************************************************************

            * Are you interested in this expertise?
            ******************************************************************************************
            Please contact CPPT UK

            Web: www.cppt.cuni.cz/ [ URL "https://cppt.cuni.cz/"]
            Mail: transfer@cuni.cz
            Phone: +420 224 491 255






            ******************************************************************************************
            * Experts and their Department

            ******************************************************************************************
            Jan Nevoral, Ph.D., M.Sc.
            Biomedical Center

            Web: http://www.biomedic-plzen.cz/cz [ URL "http://www.biomedic-plzen.cz/cz"]