Laboratory of Quantitative Histology ****************************************************************************************** * ****************************************************************************************** ****************************************************************************************** * Offer ****************************************************************************************** - Histological sectioning for routine stains as well as for immunohistochemistry (coated s - Identifying and testing continuous variables for quantification and morphometry of tissu according to the biological questions of our research partners (pilot studies with small s well as full studies) - Analyzing the sources of biological variability between groups of histological samples a large tissue samples or even whole organs by comparing tissue samples representing the sam comparing series of spatially correlated histological sections - Performing pilot and feasibility studies for sample size calculation and to find out the differences between groups of experimental animals - Evaluating sampling strategies by using systematic uniform random sampling at the level histological sections and microscopic image fi lds; calculating the sampling error and sug efficient sampling strategy for histological study - Assessment of bright field and fluorescence-labelled histological samples delivered to o - Quantification of numerical density, quantification of length, surface area, volume of h objects using unbiased stereological methods; microvessel density, area and volume fractio phases, clustering of objects, tortuosity of linear structures (fibres, microvessels) - Quantitative assessment of photomicrographs, laser scanning confocal microscope stacks f material research including tissues and bioengineered scaffolds - Basic parametric and non-parametric statistics based on data from quantitative histologi testing hypotheses using t-test, ANOVA, Mann-Whitney U test, Wilcoxon matched pairs test, ANOVA, Friedman ANOVA, correlation analysis - Writing research reports on quantitative histology using methods, photomicrographs and s of tissue samples analysis according to standards of peer-reviewed journals ****************************************************************************************** * Research Area & Excellence ****************************************************************************************** The laboratory deals with design-based unbiased stereological techniques applied for quant in histopathology. Tissue blocks and organs are processed and analysed using systematic un sampling of blocks, sections, and fields of view. The morphometry is performed using continuous variables. Thus, groups of samples may be compared in an unbiased way using standard statistical procedures for testing vario hypotheses. ****************************************************************************************** * Members ****************************************************************************************** - Assoc. Prof. Zbyněk Tonar, M.D., Ph.D., M.Sc. – Research Group Leader - Jiřina Havránková, B.Sc. - Yaroslav Kolinko, Ph.D., M.Sc. - Tereza Kubíková, M.Sc. - Anna Malečková, M.D. - Markéta Šlajerová, M.Sc. ****************************************************************************************** * Selected Publications ****************************************************************************************** - Houdek K, Moláček J, Třeška V, Křížková V, Eberlová L, Boudová L, Nedorost L, Tolinger P Kobr J, Baxa J, Liška V, Witter K, Tonar Z. Focal histopathological progression of porcine abdominal aortic aneurysm is mitigated by atorvastatin. International Angiology. 2013; 32: - Nedorost L, Uemura H, Furck A, Saeed I, Slavik Z, Kobr J, Tonar Z. Vascular histopatholo pulmonary artery banding in an in vivo growing porcine model. Pediatric Cardiology. 2013; - Eberlová L, Tonar Z, Witter K, Křížková V, Nedorost L, Korabečná M, Tolinger P, Kočová J Třeška V, Houdek K, Moláček J, Vrzalová J, Pešta M, Topolčan O, Valenta J. Asymptomatic ab aneurysms show histological signs of progression: a quantitative histochemical analysis. P 2013; 80: 11–23 - Plencner M, East B, Tonar Z, Otáhal M, Prosecká E, Rampichová M, Krejčí T, Litvinec A, B A, Nečas A, Hoch J, Amler E. Abdominal closure reinforcement by using polypropylene mesh f with poly-?-caprolactone nanofibers and growth factors for prevention of incisional hernia International Journal of Nanomedicine 2014; 9: 3263–3277 - Veselá P, Tonar Z, Sálek D, Vokurka S, Trněný M, Kodet R, Moulis M, Kašparová P, Vernero Stříteský J, Michal M, Boudová L. Microvessel density of mantle cell lymphoma. A retrospec prognostic role and the correlation with the Ki-67 and the mantle cell lymphoma internatio index in 177 cases. Virchows Archive. 2014; 465: 587–597 ****************************************************************************************** * Are you interested in this expertise? ****************************************************************************************** Please contact CPPT UK Web: www.cppt.cuni.cz/ [ URL "https://cppt.cuni.cz/"] Mail: transfer@cuni.cz Phone: +420 224 491 255 ****************************************************************************************** * Experts and their Department ****************************************************************************************** Assoc. Prof. Zbyněk Tonar, M.D., Ph.D., M.Sc. Department of Histology and Embryology Web: http://www.biomedic-plzen.cz/cz [ URL "http://www.biomedic-plzen.cz/cz"]