Laboratory of Experimental Cardiology












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            *
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            "The laboratory is focused on physiology and pathophysiology of the heart."






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            * Offer

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            - Development of experimental models and analysis of cardiac function; development of an a

            animal model (broad experience with various experimental animal species and models, e.g. r
            renal failure, rat with diabetes mellitus, rat with autonomic denervation, hypercholestero
            Carlsson’s model of rabbit, pig with sepsis) with a detailed description of cardiac functi

            variability analysis, measured both in vivo and in isolated heart, measurement of contract
            membrane potential in multicellular preparation, measurement of membrane ionic currents in
            myocytes).

            - Description and analysis of cardiac effects of substances/ drugs; pharmacological effect
            in isolated heart, in multicellular cardiac preparation, in isolated cardiac myocytes; var
            animal species (rat, rabbit, pig); analysis of electrophysiological and contractile effect

            - Testing cardiac safety of substances / drugs; analysis of contractile, electrophysiologi
            proarrhythmic effects; in vivo and in vitro testing.






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            * Expertise
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            The cardiac function is analyzed in various animal models using a number of experimental a
            several levels of biological complexity:
            - from in vivo measurements (ECG analysis) to

            - molecular level (measurement of membrane ionic currents).





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            * Members
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            - Assoc. Prof. Milan Štengl, M.D., Ph.D. – Research Group Leader

            - František Barták, M.Sc.
            - Jaroslava Hesová
            - Lucie Jacenková, M.Sc.

            - Dagmar Jarkovská, Ph.D., M.Sc.
            - Eliška Mistrová, M.D.
            - Lukáš Nalos, M.D., Ph.D.

            - Assoc. prof. Jitka Švíglerová, M.D., Ph.D.





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            * Selected Publications
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            - Stengl M, Ledvinova L, Chvojka J, Benes J, Jarkovska D, Holas J, Soukup P, Sviglerová J,

            Effects of clinically relevant acute hypercapnic and metabolic acidosis on the cardiovascu
            experimental porcine study. Crit Care 2013; 17: R303
            - Svíglerová J, Kuncová J, Nalos L, Holas J, Tonar Z, Rajdl D, Stengl M.: Cardiac remodeli

            renal failure shows interventricular differences. Exp Biol Med 2012; 237: 1056–67
            - Stengl M, Bartak F, Sykora R, Chvojka J, Benes J, Krouzecky A, Novak I, Sviglerova J, Ku
            Matejovic M.: Reduced Ltype calcium current in ventricular myocytes from pigs with hyperdy

            shock. Crit Care Med 2010, 38: 579–87
            - Kuncová J, Svíglerová J, Kummer W, Rajdl D, Chottová-Dvoráková M, Tonar Z, Nalos L, Sten
            Parasympathetic regulation of heart rate in rats after 5/6 nephrectomy is impaired despite

            intact cardiac vagal innervation. Nephrol Dial Transplant 2009; 24: 2362–70
            - Stengl M, Sykora R, Krouzecky A, Chvojka J, Novak I, Varnerova V, Kuncova J, Nalos L, Sv
            J, Matejovic M.: Continuous hemofiltration in pigs with hyperdynamic septic shock affects 

            repolarization. Crit Care Med 2008; 36: 3198–204





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            * Are you interested in this expertise?
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            Please contact CPPT UK

            Web: www.cppt.cuni.cz/ [ URL "https://cppt.cuni.cz/"]
            Mail: transfer@cuni.cz
            Phone: +420 224 491 255






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            * Experts and their department

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            Assoc. Prof. Milan Štengl, M.D., Ph.D.
            Biomedical Center

            Web: http://www.biomedic-plzen.cz/cz [ URL "http://www.biomedic-plzen.cz/cz"]