Laboratory of Bioanalytical Methods ****************************************************************************************** * ****************************************************************************************** "Our mission is to develop fast, sensitive and simple bioanalytical LC methods with the ma simple sample preparation procedure and all pre-analysis." ****************************************************************************************** * Offer ****************************************************************************************** - Development of modern bioanalytical methods made to order - Determination of vitamins in various biological matrices - Determination of pharmaceuticals in human fluids - Determination of important biomarkers - Handling and pretreatment of various biofluids as breast milk, erythrocytes, lipoprotein whole blood, wounds liquids, exudates, ascites, urine, amniotic fluid etc. - Stability studies - Method validation (FDA, ICH, PhEu, USP) - Development of modern sample preparation techniques suitable for large sequences - Sample storage ****************************************************************************************** * Expertise ****************************************************************************************** *========================================================================================= * Chromatography in Bioanalysis *========================================================================================= Research team guarantees: - correct sampling and proper storage, modern sample preparation techniques - perfect processing of biological materials, such as urine serum, plasma, erythrocytes, b exudates, lipoproteins etc. - highly qualified application of bioanalytical separation methods (HPLC, UHPLC, ultracent - proper composition of validation protocol and implementation of new methods into the pra studies, laboratory methods investigation - the obtained results publishing at the local and international level ****************************************************************************************** * Research Area & Excellence ****************************************************************************************** High Performance Liquid Chromatography (HPLC) and Ultra High Performance Liquid Chromatogr modern separation techniques, which found their important role in bioanalysis. Studies in usually contain many samples and new fast LC techniques in combination with simple pre-ana the solution. ****************************************************************************************** * Key Research Equipment ****************************************************************************************** - UFLC Nexera system equipped with the autosampler for microtitration plates and with UV-V fluorescence detector and ultra-fast mass spectrometry LCMS 80-30 detector (Shimadzu, Japa - HPLC set Prominence LC20 with DAD and fluorescence detector (Shimadzu, Japan) - Ultracentrifuge Optima Max-XP (Beckman Coulter, USA) - Sample preparation equipment: manifolds for solid phase extraction (SPE) cartridges and microtitration plates, concentrators, hood, refrigerated centrifuges, two microcentrifuges °C (long-term sample storage) ****************************************************************************************** * Main Projects ****************************************************************************************** *========================================================================================= * Analytes *========================================================================================= - Vitamins (A, D, E, B) and its metabolites, neopterin, kynurenine,´ tryptophan, creatinin deoxyguanosine, 8-hydroxyguanosine, arginine, ornithine, citruline, vancomycine *========================================================================================= * Matrices *========================================================================================= - Serum, plasma, whole blood, saliva, erythrocytes, cell lines, lipoproteins, urine, breas liquids, exudates, pleural effusions, amniotic fluid *========================================================================================= * Patients *========================================================================================= - Suffering from various types of cancer, after surgeries, gerontological and gynecologica infants, patients with metabolic diseases etc. ****************************************************************************************** * Partnerships & Collaborations ****************************************************************************************** *========================================================================================= * International collaborations *========================================================================================= - University of Coimbra – Portugal - University of Porto – Portugal - University of Tasmania – Australia - University Las Palmas – Spain - Cardiff University – Great Britain - The University St. Andrews – Great Britain - Stockholm University – Sweden *========================================================================================= * National Collaborations *========================================================================================= - Palacký University and University Hospital – Olomouc - Inst Hematol & Blood Transfus – Prague - Contipro group Ltd. – Dolní Dobrouč - St. Ann Hospital – Brno ****************************************************************************************** * Achievements ****************************************************************************************** *========================================================================================= * Publications *========================================================================================= More than 200 publications in international journals with impact factors in the field of a chemistry and medicine *========================================================================================= * Cooperating Laboratory *========================================================================================= Research laboratory at Department of Clinical Biochemistry and Diagnostic RNDr. Lenka Kujovská Krčmová, PhD. +420 495 833 372 | lenka.krcmova@faf.cuni.cz Sokolská 581, Hradec Králové, CZ-50005, CZECH REPUBLIC, EU ****************************************************************************************** * Zajímá vás tato expertíza? ****************************************************************************************** Kontaktujte CPPT UK Web: www.cppt.cuni.cz/ [ URL "https://cppt.cuni.cz/"] Mail: transfer@cuni.cz Tel.: +420 224 491 255 ****************************************************************************************** * Naši experti a jejich pracoviště: ****************************************************************************************** Prof. RNDr. Petr Solich, CSc. Department of Analytical Chemistry Web: https://portal.faf.cuni.cz/Groups/Analytical-Chemistry-Group/ [ URL "https://portal.f Groups/Analytical-Chemistry-Group/"]