Yeast Colony Group ****************************************************************************************** * ****************************************************************************************** ****************************************************************************************** * Offer ****************************************************************************************** We offer our expertise related to molecular and cellular biology of yeast, with an emphasi investigation and environmental resistance of multicellular populations such as biofilms, natural yeast strains, immobilised populations. ****************************************************************************************** * Know-how & Technologies ****************************************************************************************** - The development and differentiation of yeast colonies, a model of simple multicellular o - The function and regulation of processes involved in development of specialized colonial cells of solid tumors of mammals - The differentiation in structured biofi lm colonies of natural yeast strains - The regulation of mechanisms involved in resistance of yeast biofilm colonies and biofi environmental attack (including drug treatment) - The development of new approaches to study multicellular structures *========================================================================================= * We are looking for cooperation with public and private organizations in the field of mol and microbiology related to analyses of multicellular yeast populations. *========================================================================================= ****************************************************************************************** * Main Capabilities ****************************************************************************************** - Genetic, molecular biology and biochemistry techniques, including yeast strain modifi ca construction of knock-out strains, genomic fusions with gene tags etc.), DNA, RNA and prot microarrays, amino acid quantification (HPLC), different cellular staining, flow-cytometry - Special techniques for studies of multicellular populations including two-photon excitat microscopy for in situ analyses of population architecture and differentiation, cell micro separation and others. ****************************************************************************************** * Key Research Equipment ****************************************************************************************** Microscopy and macroscopy specialized for the investigation of multicellular communities, equipment for molecular and cellular biology, biochemistry and microbiology. ****************************************************************************************** * Partners and Collaborations ****************************************************************************************** *========================================================================================= * Academic Partners *========================================================================================= Institute of Microbiology of the CAS (Laboratory of Cell Biology; Prague, CZ) | Oslo Unive (Ullev?l) (Dept. Medical Genetics; Oslo, Norway) | Institute Jacques Monod CNRS, Paris, Fr of Tennessee, Memphis, USA | COST network “Proteostasis” BM 107 *========================================================================================= * Private and Public Sector *========================================================================================= Lenticats (CZ) ****************************************************************************************** * Main Projects ****************************************************************************************** - 15-08225s, Metabolic pathways and regulatory mechanisms responsible for vitality and lon specifically localized within yeast colonies, Grant Agency of the Czech Republic, 2015–201 - 7F14083, 3D yeast colony genomics: A model for cancer progression and development of dru biofilms, Czech-Norwegian Research Programme (CZ09), 2014–2017. - 13-08605S, Biofi lm colonies of wild yeast: Development, defense strategies and regulato Grant Agency of the Czech Republic, 2013–2017. - TA01011461, Immobilized yeasts in biotechnology: development of new applications for man Technology Agency of the Czech Republic, 2011–2014. - 204/08/0718, Role of ammonia signalling in yeast colony differentiation, development, ag Molecular mechanisms and functions, Grant Agency of the Czech Republic, 2008–2012. - 55005623, Multicellular yeast Communities: Signalling, Differentiation and Long-Term Sur Hughes Medical Institute International Research Scholar Award, USA, 2006–2011. - LC06063, Fluorescence microscopy in biological and medical research, Ministry of Educati Republic, 2006–2011 - LC531, Centre on molecular biology and physiology of yeast communities, Ministry of Educ Czech Republic, 2005–2011. - EMBO Young Investigator Award 2001 (YIP141) (EMBO/HHMI Young Investigator 2003–2005). “S involved in the development of multicellular structures – yeast colonies”. ****************************************************************************************** * Achievements ****************************************************************************************** Czech patent: CZ 305223 „Method of modifi cation of the detection yeast strain“ (2015) and peer-reviewed and high-impact journals such as Nature, J Cell Biol, J Cell Sci, Mol Biol C FEMS Microbiol Rev, EMBO Rep, Env Microbiol and Mol Cell. Results are regularly presented invited lectures at leading international conferences and in prestigious Universities. ****************************************************************************************** * Are you interested in this expertise? ****************************************************************************************** Please contact CPPT UK Web: www.cppt.cuni.cz/ [ URL "https://cppt.cuni.cz/"] Mail: transfer@cuni.cz Phone: +420 224 491 255 *========================================================================================= * Experts and their Department *========================================================================================= Prof. RNDr. Zdena Palková, CSc. Department of Genetics and Microbiology Web: web.natur.cuni.cz/~zdenap [ URL "http://web.natur.cuni.cz/~zdenap"]