Mitochondrial Laboratory












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            * Offer
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            - Isolation of mitochondria from fresh tissues and cells, optimization of isolation protoc

            of the quality of isolated organelles
            - Preparation of protocols for optimum tissue permeabilization and homogenization

            - Analysis of mitochondrial respiratory activity in biological samples using high-resoluti
            - Determination of activity of selected mitochondrial enzymes
            - Evaluation of the effect/s of experimentally induced pathological states and pharmacolog

            mitochondrial respirátory parameters in animal studies
            - Analysis of mitochondrial activity in fresh human cells (platelets, spermatozoa) or huma
            - Statistical analysis of the collected data for publication purposes, testing of hypothes

            analysis





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            * Expertise
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            - The Mitochondrial Laboratory focuses on evaluation of respiratory activity of isolated m

            mitochondria in situ, i.e. in tissues or cells aft er mechanical and/or chemical permeabil
            - The method of high-resolution respirometry enables analysis of individual complexes of m
            respiratory system in tissue biopsies and intact or permeabilized cells (cultured cell lin

            spermatozoa).





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            * Members
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            - Assoc. Prof. Jitka Kuncová, M.D., Ph.D. – Research Group Leader

            - Miroslava Čedíková, Ph.D., M.D.
            - Michaela Marková, M.Sc.
            - Renata Šťastná






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            * Selected Publications

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            - Cedikova M, Miklikova M, Stachova L, Grundmanova M, Tuma Z, Vetvicka V, Zech N, Kralicko
            Effects of the Czech propolis on sperm mitochondrial function. Evid Based Complement Alter

            2014: 248768
            - Svíglerová J, Kuncová J, Stengl M. Contractile functions of myocardium and their regulat
            2014; 63 (1): 25–33

            - Cedíková M, Miklíková M, Grundmanová M, Zech NH, Králíčková M, Kuncová J. Sperm mitochon
            men with normozoospermia and asthenozoospermia. Ceska Gynekol. 2014; 79 (1): 22–8
            - Pfeil U, Kuncova J, Brüggmann D, Paddenberg R, Rafiq A, Henrich M, Weigand MA, Schlüter 

            Middendorff R, Slavikova J, Kummer W. Intrinsic vascular dopamine - a key modulator of hyp
            vasodilatation in splanchnic vessels. J Physiol. 2014; 592 (Pt 8): 1745–56
            - Svíglerová J, Kuncová J, Nalos L, Holas J, Tonar Z, Rajdl D, Stengl M. Cardiac remodelin

            renal failure shows interventricular differences. Exp Biol Med (Maywood). 2012; 237 (9): 1
            - Kochová P, Kuncová J, Svíglerová J, Cimrman R, Miklíková M, Liška V, Tonar Z. The contri
            vascular smooth muscle, elastin and collagen on the passive mechanics of porcine carotid a

            Meas. 2012; 33 (8): 1335–51
            - Švíglerová J, Mudra J, Tonar Z, Slavíková J, Kuncová J. Alteration of the cardiac sympat
            is modulated by duration of diabetes in female rats. Exp Diabetes Res. 2011; 2011: 835932

            - Kuncová J, Chvojka J, Sýkora R, Svíglerová J, Stengl M, Nalos L, Kroužecký A, Matějovič 
            concentrations of vasoactive intestinal peptide are affected by peritonitis-induced sepsis
            hemofiltration in pigs. Physiol Res. 2011; 60 (3): 531–40

            - Kuncová J, Sýkora R, Chvojka J, Svíglerová J, Stengl M, Kroužecký A, Nalos L, Matějovič 
            tissue levels of neuropeptide y in experimental septic shock: relation to hemodynamics, in
            oxidative stress, and hemofiltration.

            Artif Organs. 2011; 35 (6): 625–33
            - Svíglerová J, Kuncová J, Nalos L, Tonar Z, Rajdl D, Stengl M. Cardiovascular parameters 
            chronic renal failure induced by subtotal nephrectomy. Physiol Res. 2010; 59 Suppl 1: S81–






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            * Are you interested in this expertise?

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            Please contact CPPT UK
            Web: www.cppt.cuni.cz/ [ URL "https://cppt.cuni.cz/"]

            Mail: transfer@cuni.cz
            Phone: +420 224 491 255






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            * Experts and their department
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            Assoc. Prof. Jitka Kuncová, M.D., Ph.D.
            Institute of Physiology
            Web: www.biomedic-plzen.cz [ URL "http://www.biomedic-plzen.cz"]