Laboratory of Molecular Genetics of Development












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            "We strive to understand the dynamic process of vesicular trafficking by studying the intr

            interactions at the endosomal surface."





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            * Offer
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            We are looking for meaningful partnerships with academic and private institutions. The qui
            to discuss a partnership with us is to contact us and ask. We prefer meaningful discovery-
            collaborations with responsible and reliable partners.


            We have considerable expertise in the following:
            - Characterisations of intracellular trafficking pathways of your protein or drug of inter

            the cargo inside cells using various microscopic approaches
            - Genetic studies in C. elegans – using our library of siRNA, we are able to study the eff
            silencing on physiological processes in C. elegans






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            * Know-how & Technologies

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            The two models used in our laboratory are human cell lines and the “worm” – Caenorhabditis
            findings in one of the models can be quickly verified in the other – human cell lines prov

            relevance while the worm is an excellent and easy-to-grow animal model to study the influe
            pathways on organ development.


            Our experimental work combines two principal approaches: the fluorescent microscopic obser
            and proteomic work. Microscopy, including advanced live imaging and super-resolution techn
            to understand the localisation of proteins of interest and their transport across cells. W

            pinpoint proteins to precise vesicular subcompartments inside cells. Our biochemical metho
            the studies of protein-protein interactions including mass spectrometry.






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            * Key Research Equipment
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            As part of the Department of Cell Biology, we have easy access to a wide array of laborato
            This includes the Laboratory of Confocal and Fluorescent Microscopy, where we regularly op
            Scan^R wide-fi eld microscope,

            Zeiss LSM 880 confocal microscope and Zeiss Elyra SP.1 superresolution microscope. We also
            use of the laboratory of cytometry and laboratory of mass spectrometry, the latter of whic
            system for the analysis of complex samples. Other equipment at our disposal includes a seq

            culture and C. elegans cultivation rooms as well as an ultracentrifuge, Varioskan fluorome
            system for the detection of chemiluminescence and other biochemical equipment.






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            * PartnershiPs & Collaborations
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            * Academic Partners
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            - Kateřina Schwarzerová – Faculty of Science, Charles University, Prague

            - Zdeněk Lánský – BIOCEV, Vestec u Prahy
            - Robert Insall, Laura Machesky – Beatson Institute for Cancer Research, Glasgow, Cancer R






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            * Achievements
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            - Publications in respected international journals





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            * Are you interested in this expertise?
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            Please contact CPPT UK

            Web: www.cppt.cuni.cz/ [ URL "https://cppt.cuni.cz/"]
            Mail: transfer@cuni.cz
            Phone: +420 224 491 255






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            * Experts and their department

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            Mgr. Marie Macůrková, Ph.D.
            RNDr. Lenka Libusová, Ph.D.

            Department of Cell Biology
            Web: https://www.natur.cuni.cz/biology/cell-biology/research-teams/laboratory-of-molecular
            development [ URL "https://www.natur.cuni.cz/biology/cell-biology/research-teams/laborator

            genetics-of-development"]