Laboratory of Drug Metabolism, Interactions and Resistance ****************************************************************************************** * ****************************************************************************************** "We are open to a wide spectrum of collaboration with academic partners as well as with pa applied research, industry and agronomy." ****************************************************************************************** * Offer ****************************************************************************************** *========================================================================================= * Drug Metabolism Studies in Vitro and/or in Vivo *========================================================================================= - Antiproliferative and toxicity tests using various cultures of immortalized cells - Tests of hepatotoxicity of drugs and other xenobiotics - Preparation and incubation of precision-cut liver and intestinal slices - Isolation and cultivation of primary cultures of hepatocytes - Preparation of subcellular fractions (animal and human organs, parasites, plants, cell c - Assays of enzyme expression and enzymatic activities - Identification of metabolites of drugs and other xenobiotics *========================================================================================= * Drug Resistance Studies in Helminths *========================================================================================= - In vivo animal studies - Isolation of helminths from infected hosts - Ex vivo cultivations of helminths - Coprological testing ****************************************************************************************** * Expertise ****************************************************************************************** - Drug biotransformation and transport studies in humans, animals, parasitic helminths and - Effect of drugs, dietary supplements, natural compounds and other xenobiotics on express of drug-metabolizing enzymes - Pharmacodynamics mechanisms of drug resistance in helminths ****************************************************************************************** * Research Area & Excellence ****************************************************************************************** - Identification of metabolites of drugs and other xenobiotics in humans, animals, parasit plants - Toxicity and interactions of natural substances/ food supplements with drugs - Mechanisms of drug resistance in parasitic helm inths ****************************************************************************************** * Key Research Equipment ****************************************************************************************** - Accredited vivarium - Cell and tissue laboratory - Laboratory for isolation and cultivation of helminths - Laboratory for molecular biology (electrophoresis, Western blotting, qPCR) - Analytical devices (spectrophotometers, spectrofl uorimeters, HPLC, LC/MS) ****************************************************************************************** * Achievements ****************************************************************************************** Publications in respected international journals. "Our mission is to get better knowledge of fate of drugs in various organisms and the fact affect drug efficacy and safety.“ ****************************************************************************************** * Partnerships & Collaborations ****************************************************************************************** *========================================================================================= * Main Projects *========================================================================================= - 18-08452S “Anthelmintics in plants – interactions with polyphenols biosynthesis and anti - 18-07724S “Circulation of anthelmintics in environment – does it contribute to drug resi development in helminths?“ - 18-09946S “Metabolism of terpenes and mechanisms of their toxic effects in human liver“ - 18-07281Y “In vitro and ex vivo models to study drug-drug interactions of antiviral drug transporters“ - 17-11954Y “UDP-glycosyltransferases in Haemonchus contortus – players in drug-resistance - No. P303/12/G163 “Centre of drug – dietary supplement interactions and nutrigenetics” - UNCE18/SCI/012 “Centre of research of hosts-parasites interactions“ - PRIMUS/17/SCI/4 “Úloha mikroRNA v lékové rezistenci hlístic” *========================================================================================= * Are you interested in this expertisa? *========================================================================================= Please contact CPPT UK Web: www.cppt.cuni.cz/ [ URL "https://cppt.cuni.cz/"] Mail: transfer@cuni.cz Tel.: +420 224 491 255 *========================================================================================= * Experts and their department *========================================================================================= Prof. Ing. Barbora Szotáková, Ph.D. Department of Biochemical Sciences Web: http://portal.faf.cuni.cz/Groups/Drug-resistance-and-drug-interactions [ URL "http:// portal.faf.cuni.cz/Groups/Drug-resistance-and-drug-interactions"]