Laboratory of Heme-Containing Sensor Proteins ****************************************************************************************** * ****************************************************************************************** "We are looking for cooperation in the fields of hemoprotein’s related research focused ma enzymes or heme-containing sensors." ****************************************************************************************** * Offer ****************************************************************************************** We offer our expertise, as well as consultancy or collaboration within a diverse range of clinical biochemistry related topics, particularly in the fields of: - hemoproteins (heme enzymes and heme-containing sensor proteins) - diatomic gas molecules (NO, CO and O2) and their interaction with heme in hemoproteins - enzymology - post-translation modifications of hemoproteins - protein stability and structure-function relationship - oxidative stress in cells - heavy metal ions including their complexes (such as free heme) and their effect on cells - ionizing radiation and its effect on cells ****************************************************************************************** * Know-how & Technologies ****************************************************************************************** - Identification of new hemoproteins and description of their properties - Various hemoproteins production and/or isolation, their characterization including prote - Development of new antibacterial strategies based on the research focused on heme enzyme sensors - Development of new anticancer strategies based on the research focused on heme-containin that are unique for pathological bacteria - Detection of diatomic gas molecules (NO, CO and O2) based on their specific interaction containing gas sensor proteins - Molecular modeling of hemoproteins ****************************************************************************************** * Content of Research ****************************************************************************************** *========================================================================================= * Heme-containing Sensor Proteins *========================================================================================= - structure-function relationships - involvement in carcinogenesis - anticancer therapy - involvement in bacterial pathogenesis - antibacterial therapy *========================================================================================= * Heme Enzymes *========================================================================================= - structure-function relationships - involvement in carcinogenesis – anticancer therapy *========================================================================================= * Mechanism of Signal Transduction in Eukaryotic and Prokaryotic Cells *========================================================================================= - Role of heme in regulatory pathways (for example in regulation of protein expression) ****************************************************************************************** * Main Capabilities ****************************************************************************************** Protein production, protein isolation, protein characterization, protein engineering, enzy protein-ligand interaction studies, immunochemical methods, electrochemical methods, chrom spectrophotometry ****************************************************************************************** * Key Research Equipment ****************************************************************************************** Absorption spectroscopy, equipment for various chromatography approaches, mass spectrometr equipment for molecular biology and protein engineering ****************************************************************************************** * Partnerships & Collaborations ****************************************************************************************** *========================================================================================= * Academic Partners *========================================================================================= - Ecole Polytechnique, Palaiseau, France (Marten Vos and Ursula Liebl – ultrafast spectros - Université Montpellier, France (Reinhard Lange – spectroscopy under high pressure) - Cyprus University of Technology, Limessol and University of Cypru, Nicosia, Cyprus (Cons and Eftychia Pinakoulaki – FTIR and Raman spectroscopy) - Laboratory of Pediatric Infect. Diseases, Nijmegen, Netherlands (Marien de Jonge – micro - Tohoku University, Sendai, Japan (Kazuhiko Igarashi – eukaryotic heme sensor proteins) - Institute of Microbiology, Prague, Czech Republic (Miroslav Šulc – mass spectrometry) - BIOCEV, Vestec, Czech Republic (Petr Man and Petr Pompach – mass spectrometry; Jan Dohná *========================================================================================= * Private and Public Sector *========================================================================================= - IPSEN Pharma, os. (France, 2014–2015) - BEDO (Thailand, 2015–2016) . National Radiation Protection Institute (Czech Republic, 2019–) ****************************************************************************************** * Main Projects ****************************************************************************************** - 2012–2017: Modern technologies for identification and optimization of anti-tumor drugs o generation. Participation in the project granted to Assoc. Prof. RNDr. Miroslav Šulc, PhD. - 2014–2015: Comparison of the adsorption capacity and trapping effectiveness of diosmecti on the compounds causing the 10 most frequent intoxications in acute medicine. External sp IPSEN Pharma, os. - 2015–2017: Molecular mechanisms of intraprotein/interdomain signal transduction in model proteins. GAČR, 15-19883S ****************************************************************************************** * Achievements ****************************************************************************************** - Stranava M, Man P, Skálová T, Kolenko P, Blaha J, Fojtikova V, Martínek V, Dohnálek J, L Rosůlek M, Shimizu T, Martinkova M (2017) Coordination and redox state-dependent structura heme-based oxygen sensor AfGcHK associated with intraprotein signal transduction, J Biol C 20935 - Mináriková M, Fojtikova V, Vyskočilová E, Sedláček J, Šikut M. Borek-Dohalska L, Stiboro M (2017) The capacity and effectiveness of diosmectite and charcoal in trapping the compou most frequent intoxications in acute medicine: a comparative study, Environ Toxicol Phar 5 - Stranava M, Martinek V, Man P, Fojtikova V, Kavan D, Vaněk O, Shimizu T, Martinkova M (2 Characterization of the Heme-based Oxygen Sensor, AfGcHK, its Interactions with the Cognat Regulator, and their Combined Mechanism of Action in a Bacterial Two-component Signaling S 84: 1375–89 - Kuipers K, Gallay C, Martínek V, Rohde M, Martínková M, van der Beek SL, Jong WS, Vensel Bootsma H, Veening JW, de Jonge MI (2016) Highly conserved nucleotide phosphatase essentia lipid homeostasis in Streptococcus pneumoniae. Mol Microbiol 101: 12–26 - Lambry J-Ch, Stranava M, Lobato L, Martinkova M, Shimizu T, Liebl U, Vos MH (2016) Ultra Evidence for Picosecond Ligand Exchange at the Binding Site of a Heme Protein: Heme-based Phys Chem Lett 7: 69–74 - Fojtikova V, Stranava M, Vos M H, Liebl U, Hraníček J, Kitanishi K, Shimizu T, Martinkov Kinetic Analysis of a Globin-coupled Histidine Kinase, AfGcHK: Effects of the Heme Iron Co Regulator and Metal Cations on Autophosphorylation Activity. Biochemistry 54: 5017–5029 - Shimizu T, Yan F, Huang D, Stráňava M, Bartošová M, Fojtíková V, Martínková M (2015) Mol Characteristics of Heme- based Gas (O2, NO and CO) Sensors. Chem Rev 115: 6491?6533 ****************************************************************************************** * Are you interested in this expertisa? ****************************************************************************************** Please contact CPPT UK Web: www.cppt.cuni.cz/ [ URL "https://cppt.cuni.cz/"] Mail: transfer@cuni.cz Tel.: +420 224 491 255 ****************************************************************************************** * Experts and their department ****************************************************************************************** Assoc. Prof. Markéta Martínková, Ph.D. Department of Biochemistry Web: www.natur.cuni.cz/martinkova [ URL "http://www.natur.cuni.cz/martinkova"]