Laboratory of Cancer Cell Invasion ****************************************************************************************** * ****************************************************************************************** "To elucidate the mechanisms of cancer cell invasiveness, identify novel targets for anti- treatment and help to develop antimetastatic drugs – migrastatics." ****************************************************************************************** * Offer ****************************************************************************************** - Our expertise in quantitative analysis of cell migration and invasiveness in various 2D environments under any selected conditions - Suitable targets and – in cooperation – suitable drug candidates for anti-metastatic tre ****************************************************************************************** * Requirement ****************************************************************************************** - All kind of cooperation in field of cancer cell invasiveness - Collaborations on translation of our anti-metastatic drug candidates into clinics ****************************************************************************************** * Know-how & Technologies ****************************************************************************************** - Identification and understanding of proteins and signaling pathways involved in amoeboid cancer cell invasiveness - Elucidation of the mechanisms of plasticity of cancer cell invasiveness - Functional analysis of subcellular structures involved in cancer cell invasiveness - Analysis of general mechanisms of cell migration and mechanosensing The focus of our laboratory is on molecular and cellular mechanisms of cancer cell motilit invasiveness and its plasticity, including subcellular structures. We discovered and chara invasiveness and metastasis in cancer cells of mesenchymal origin. We were the fi rst to e structure of invadopodia in a complex 3D environment. Later, we concentrated more on plast cell invasiveness and succeeded in identification of several important signaling molecules process. ****************************************************************************************** * Main Capabilities ****************************************************************************************** - Wide range of basic and advanced genetic, molecular biology and biochemistry techniques CRISPR/Cas9, shRNA, affinity purifications, kinase assays and many others) - Cell culture in 2D and 3D conditions on various matrices (including acellular dermis, Ma collagen, cell-based matrices) - Transcriptomic and proteomic analyses in 3D collagen - Qualitative and quantitative cell adhesion, proliferation, migration, ECM degradation, i formation and invasion assays in 2D and 3D environments - Advanced microscopic analyses of migrating cancer cells as well as signaling proteins an involved in cell adhesion, migration and invasiveness ****************************************************************************************** * Key Research Equipment ****************************************************************************************** All standard facilities required for molecular biology and biochemistry, cell culture faci box, fluorescent microscopes (Nikon Eclipse TE2000S, JuLi), xCELLigence. As a part of the the Cell Invasion in Cancer laboratory has access to all facilities at BIOCEV including Ad facility to cover all required microscopy techniques (superresolution, correlation, spinni Leica TCS SP8 WLL SMD-FLIM, Super-resolution Nikon Ti-E microscopes with N-SIM and N-STORM Nikon Ti-E with STED module from Abberior Instruments, Carl Zeiss LSM 880 NLO (Intravital photon and confocal microscope), FEI Helios NanoLab G3 UC–FIB-SEM equipped for CLEM plus a sample preparation. The proteome or metabolome analysis and quantification on the latest g Mass spectrometry equipment–Thermo Orbitrap Fusion is also available at BIOCEV. The resear access to the Faculty of Science Laboratory of Electron Microscopy (JEOL JEM-1011 TEM, JEO and all other necessary equipment). ****************************************************************************************** * Partnerships & Collaborations ****************************************************************************************** *========================================================================================= * Academic Partners *========================================================================================= Ben Fabry (FAU, Erlangen-Nürnberg, biophysics), Karel Smetana Jr. (Charles University, tum microenvironment), Victoria Sanz-Moreno (King´s College, plasticity of cancer cell invasiv Weaver (Vanderbilt University, invadopodia), Stephen J. Weiss (University of Michigan, pro cell motility), Daniel Zícha (CEITEC, advanced microscopy) *========================================================================================= * Private and Public Sector *========================================================================================= Medicem (acellular dermis), Medbase (clinical translation) *========================================================================================= * Main Projects *========================================================================================= - GA ČR grant 18-15684J The role of matrix metalloproteinases and vimentin cooperation in invadopodia function - GA ČR grant 15-07321S Analysis of Src kinase activation using a novel FRET-based biosens - GA ČR grant 15-174195 Crosstalk of CAS/BCAR1 and PKN3 signaling in invasiveness and meta cells - KFF PI Grant The Kellner Family Foundation grant The analysis of plasticity of cancer ce - GA ČR grant 13-24851J Dissecting of BCAR1/CAS role in mechanosensing ****************************************************************************************** * Achievements ****************************************************************************************** Publications in peer-reviewed and high-impact journals such as Oncogene, Molecular Cancer of Proteome Research, Molecular Carcinogenesis, Cellular and Molecular Life Sciences, Mole of the Cell, Oncotarget, Lancet Oncology, Journal of Cell Science, New England of Medicine are regularly presented as invited lectures at leading international conferences and at pr universities. ****************************************************************************************** * Are you interested in this expertise? ****************************************************************************************** Please contact CPPT UK Web: www.cppt.cuni.cz/ [ URL "https://cppt.cuni.cz/"] Mail: transfer@cuni.cz Phone: +420 224 491 255 ****************************************************************************************** * Experts and their department ****************************************************************************************** Assoc. Prof. RNDr. Jan Brábek, Ph.D. Assoc. Prof. RNDr. Daniel Rösel, Ph.D. Department of Cell Biology Web: www.natur.cuni.cz/biology/cell-biology/research-teams/skupinainvazivity-nadorovych-bu "http://www.natur.cuni.cz/biology/cell-biology/research-teams/skupinainvazivity-nadorovych