Laboratory of Antibiotic Resistance and Applications of Mass Spectrometry in Microb ****************************************************************************************** * ****************************************************************************************** ****************************************************************************************** * Offer ****************************************************************************************** - DNA sequencing, including capillary and whole-genome sequencing, and metagenomic analysi - Molecular typing of Gram-negative bacteria - Detection of resistance mechanisms in Gram-negative bacteria (?-lactamases, mechanisms o fluroquinolones, aminoglycosides, colistin, etc.) - Applications of mass spectrometry in microbiology - Applications of Raman spectroscopy in microbiology ****************************************************************************************** * Expertise ****************************************************************************************** - The research projects aim to execute molecular epidemiology of factors affecting dissemi drug resistance among nosocomial pathogens. - Methodological approaches, also, include analysis of genetic carriers of resistance dete whole-genome sequencing. - The second main objective of the research projects is intending in the development of ne the detection and study of resistance mechanisms using MALDI-TOF mass spectrometry and Ram ****************************************************************************************** * Research Area & Excellence ****************************************************************************************** Scientific activities of Laboratory of Antibiotic Resistance and Application of Mass spect Microbiology (ARAMM) are mainly focused on mechanisms of resistance to antibiotics, especi spectrum cephalosporins and carbapenems, in Gram-negative bacteria (Enterobacteriaceae, Ps In 2011, the members of our group demonstrated that MALDI-TOF mass spectrometry can direct carbapenemase activity. Also, recently, we patented a method for the identification of ?-l clinical isolates by MALDI-TOF mass spectrometry, based on their molecular weight. ****************************************************************************************** * Members ****************************************************************************************** - Assoc. Prof. Jaroslav Hrabák, Ph.D., M.Sc.– Research Group Leader - Constantinos C. Papagiannitsis, Ph.D. - Kateřina Chudějová, M.Sc. - Anna Skálová, M.D. - Dana Králová, DiS. - Tamara Bergerová, M.D. - Ibrahim Bitar, Ph.D. - Miroslava Chalupová, M.D. - Vladislav Jakubů, M.Sc. - Helena Janouškovcová, M.D. - Lucie Kraftová, B.Sc. - Veronika Pašková, M.Sc. - Radka Walková, M.D. - Assoc. Prof. Helena Žemličková, M.D., Ph.D. ****************************************************************************************** * Selected Publications ****************************************************************************************** - Hrabák J., Papagiannitsis C.C., Chudáčková E.: Detection of carbapenemases in Enterobact challenge for diagnostic microbiological laboratories. Clinical Microbiology and Infection 853. DOI: 10.1111/1469-0691.12678 - Papagiannitsis C.C., Kotsakis S.D., Tuma Z., Gniadkowski M., Miriagou V., Hrabak J.: Ide CMY-2-type cephalosporinases in clinical isolates of Enterobacteriaceae by MALDI-TOF MS. A Agents and Chemotherapy 58 (5), 2014: 2952–2957. DOI: 10.1128/AAC.02418-13 - Hrabák J., Papagiannitsis C.C., Študentová V., Jakubů V., Fridrichová M., Žemličková H. Czech Participants of European Antimicrobial Resistance Surveillance Network: First outbre carbapenemaseproducing Klebsiella pneumoniae in the Czech Republic in 2011. Eurosurveillan pii=20626 - Papagiannitsis C. C., Studentova V., Hrabak J., Kubele J., Jindrák V., Zemlickova H.: Is a nonclinical sample of a Leclercia adecarboxylata producing a VIM-1 metallo-?-lactamase. Agents and Chemotherapy 57, 2013: 2896– 2897. DOI: 10.1128/AAC.00052-13 - Hrabák J., Studentová V., Walková R., Zemlicková H., Jakubu V., Chudácková E., Gniadkows Pfeifer Y., Perry J.D., Wilkinson K., Bergerová T.: Detection of NDM-1, VIM-1, KPC, OXA-48 carbapenemases by MALDI-TOF mass spectrometry. Journal of Clinical Microbiology 50, 2012: 10.1128/JCM.01002-12 - Hrabák J., Walková R., Študentová V., Chudáčková E., Bergerová T.: Carbapenemase Activit by Matrix-Assisted Laser Desorption/Ionisation Time-of-Flight Mass Spectrometry. Journal o Microbiology 49, 2011: 3222–3227; DOI: 10.1128/JCM.00984-11 *========================================================================================= * Patent *========================================================================================= - PV2013-473 / Nr. 304833 Method of Detection of Gram-negative Bacteria Periplasmic Space Outer Membrane Proteins by Mass Spectrometry – Czech National Patent - PV2015-847 / Nr. 307243 A method of wet deposition of a microbial culture to a solid sur the taxonomic identification of cultures by means of desorption-ionization techniques of m - US Patent 9,803,229: Method of detection of Gram-negative bacteria periplasmic space and membrane proteins by mass spectrometry *========================================================================================= * Are you interested in this expertise? *========================================================================================= Please contact CPPT UK Web: www.cppt.cuni.cz/ [ URL "https://cppt.cuni.cz/"] Mail: transfer@cuni.cz Phone: +420 224 491 255 *========================================================================================= * Experts and their department *========================================================================================= Assoc. Prof. Ing. Jaroslav Hrabák, Ph.D. Biomedical Center Web: www.biomedic-plzen.cz [ URL "http://www.biomedic-plzen.cz"]