Center of Advanced Preclinical Imaging ****************************************************************************************** * ****************************************************************************************** ****************************************************************************************** * Offer ****************************************************************************************** - The Center of advanced preclinical imaging (CAPI) offers collaborations implementing hig imaging technologies of with small laboratory animals in basic and applied research - The center is equipped with anatomical and functional/molecular in vivo imaging techniqu - The center enables multimodal imaging by combining different imaging modalities - We are open to collaborate with academic and scientific teams in the Czech Republic and as well as with commercial partners - The center enables small laboratory animal housing during longitudinal studies in its ow facility. Animals (laboratory mice or rats) are kept in individually ventilated cages (IVC - The center is able to provide housing for small laboratory animals during longitudinal s (laboratory mice and rats) are kept in individually ventilated cages (IVC) - Organizing of workshops, seminars - Excursions, practical classes, study visits, research fellowships at our facility ****************************************************************************************** * Know-how & Technologies ****************************************************************************************** - Preclinical in vivo studies with small laboratory animals (rodents) - Evaluation of effects, pharmacokinetics, and pharmacodynamics of applied drugs - Development and testing of new multimodal contrast agents, fluorescent and radiologic pr - Quantification of radiopharmaceutical distribution in particular organs and tissues - Multimodal examination or imaging of small laboratory animals (mice, rats) by X-ray, CT, SPECT, US, optical imaging, including photoacoustics, fl uorescence, luminescence, and Che optical methods. In vitro cell characterisation can be supplemented by imunophenotypisation with morphological cell deter cytometry - The investigated object can be measured by all the presented methods and achieved images corregistered to provide a final complex image - All the instruments are equipped with anesthetic units and vital function monitoring sys respiration rate, body temperature) - Transplantation of labelled cells and their tracking in the tissue ****************************************************************************************** * In Vivo Imaging ****************************************************************************************** - In vivo imaging of small laboratory animals includes anatomical/ morphological as well a molecular imaging techniques used for scientific and research purposes. Anatomical methods resolution images of body structures (bones, soft tissues). They show morphology and struc of particular organs. On the contrary, molecular methods provide information about functio activity of the tissue and image labelled molecules/cells accumulation in the organism. - methods may be combined. Acquired images may be merged into a final one with a complex inf the tracked process. *========================================================================================= * Magnetic Particle Imaging - MPI (Bruker) *========================================================================================= The imager detects superparamagnetic nanoparticles using an oscillating magnetic field. Ma imaging is a quantitative tomographic 3D functional/molecular imaging technique with high (up to 46 images/s). *========================================================================================= * MRI Imager ICON (Bruker) *========================================================================================= ICON exploits a permanent magnet without the need of a cryogenic media for standard 3D MR *========================================================================================= * CT/PET/SPECT Multimodální Zobrazovač Albira (Bruker) *========================================================================================= Albira PET/SPECT/CT enables highly sensitive multimodal tomographic examination which comb CT examination with PET and SPECT imaging of radionuclides. It serves for non invasive pha (pharmacokinetics, probe accumulation in the tissue and functional aktivity) and biologica perfussion, metabolism) examinations. *========================================================================================= * Optical Imager Xtreme (Bruker) *========================================================================================= Xtreme is an in vivo imager combining 5 optical methods: bioluminiscence, multispectral VI fluorescence, direct radioisotope tracing, Cherenkov radiation and X-ray. 8 mice can be im simultaneously. The instrument is equipped with a module for 3D imaging. *========================================================================================= * Imaging Flow Cytometer AMNIS (Millipore) *========================================================================================= Imaging flow cytometer AMNIS ImageStreamX MkII combines multicolor flow cytometry with opt (microscopic) imaging. The device provides high speed, high capacity image cell analysis ( thousands in one sample). *========================================================================================= * Ultrasound and Photoacoustic Imager Vevo Lazr-X (Visualsonics) *========================================================================================= The device enables high frequency ultrasound in vivo examination with 30 um resolution. Ph pigments (oxygenated/ deoxygenated hemoglobin, melanin etc.) can be detected in the photoa The ultrasound and photoacoustic images can be corregistered. Photoacoustic examination is observation of tumour vascularisation, tissue perfussion, local hypoxia, detection of labe nanoparticles or cells. Measurements can be performed in 2D, 3D and 4D mode. *========================================================================================= * Spectral X-ray Imager Color-x-scan (Radalitica) *========================================================================================= Color-x-scan provides 2D and pseudo-3Dex vivo and in vivo imaging by using single photon d TimePix detector. The spectrum of the detected radiation enables to distinguish tissues, w similar contrast on standard X-ray; thus a multicolor x-ray can be obtained. High-speed 2D is also possible. The center has to its disposal other supplementary technologies, such as a relaxometer, au portable X-ray imager, operational microscope, 3d printers (fdM, sla), shielded flow-box w chambre, ivC housing facility etc. ****************************************************************************************** * Collaboration ****************************************************************************************** - The Institute of Macromolecular Chemistry of the Academy of Sciences, Prague - The Regional Centre of Advanced Technologies and Materials (RCPTM), Faculty of Science, University, Olomouc - Biotechnology and Biomedicine Center of the Academy of Sciences and Charles University i - Department of Analytical Chemistry, UCT Prague - Prague General University Hospital - ESTHÉ – Plastic Surgery clinic, Prague - ADVACAM s.r.o. CAPI is a member of CzechBioimaging and EuroBioimaging. we were funded by projects OP VaVp ****************************************************************************************** * Are you interested in this expertise? ****************************************************************************************** Please contact CPPT UK Web: www.cppt.cuni.cz/ [ URL "https://cppt.cuni.cz/"] Mail: transfer@cuni.cz Phone: +420 224 491 255 ****************************************************************************************** * Experts and their department ****************************************************************************************** RNDr. Luděk Šefc, CSc. Center of Advanced Preclinical Imaging Web: http://capi.lf1.cuni.cz/en [ URL "http://capi.lf1.cuni.cz/en"]