Laboratory of Plant Biochemistry












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            "Our main goal is elucidating rearrangements of metabolic pathways during abiotic and biot

            plants."





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            * Offer
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            We offer our expertise, as well as consultancy or collaboration within a diverse range of 
            biochemistry and biotechnology.


            - Enzyme assays
            - Enzyme kinetics
            - Inhibition studies

            - Determination of antioxidant capacity
            - Electrophoretic separations of enzymes
            - Immunochemical assays, ELISA, Western blot






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            * Know-how & Technologies

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            * Applications of Stress Factors and Their Quantifications
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            - Biotic stress caused by Potato virus Y, tobacco mosaic virus (virus quantification – ELI
            qPCR)
            - Salt stress (atomic absorption spectrometry)

            - Drought stress (relative water content)
            - Phytoremediation of pharmaceuticals
            - Nutrition, organic source of N (analysis of medium composition and enzyme activities)

            - Heat stress (Heat shock protein quantification)




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            * Time Course of Stress Treatment

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            - Physiological and photosynthetic parameters
            - Enzyme activities: NADP-dependent enzymes, Hatch-Slack cycle enzymes, enzymes of N, C me

            GS, GOGAT, GDH, glycosidases, ?-fructosidase, amylase, cellulase, chitinase, ?-1,3 glucana
            enzymes (SOD, peroxidases, catalase, glutathione reductase)




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            * Enzyme Isolation and Characterization, Inhibition Studies
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            - NADP-malic enzyme

            - Phosphoenolpyruvate carboxylase
            - ?-hexosaminidase
            - Shikimate dehydrogenase

            - Alcohol dehydrogenase
            - Lactate dehydrogenase






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            * Content of Research
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            - Abiotic plant stress (drought, salinity, xenobiotics, high temperature)
            - Biotic plant stress (viral infection)
            - Plant nutrition, organic sources of nitrogen

            - Biological control agents for sustainable agriculture
            - Screening of antioxidant activities in plants




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            * Main Capabilities
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            - Plant Biochemistry

            - Enzymology
            - Stress markers
            - Plant nutrition and in vitro cultivation






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            * Key Research Equipment

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            - Spectrophotometer UV-VIS Helios alpha Thermo Spectronic, USA
            - Spectrophotometer UV-VIS Ultrospec 2100 ProBiochrom, UK

            - Trans-Blot Turbo Transfer System Bio Rad, USA
            - Electrophoretic Separation System, Biometra, Germany
            - Microplate Reader Multiskan Go, Thermo Scientific, USA

            - Microplate Reader Infinite M200 pro, Tecan, USA
            - CFX Connect Real Time Detection System, Bio Rad, USA






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            * Partnerships & Collaborations
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            * Academic Partners
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            Institute of Experimental Botany Academy of Sciences of the Czech Republic | Institute of 

            Academy of Sciences of the Czech Republic | CEITEC — Central European Institute of Technol
            University in Brno | BIOCEV — Biotechnology and Biomedicine Centre of the Academy of Scien
            University | Institute of Organic Chemistry and Biochemistry of the Czech Academy of Scien

            University, Diepenbeek, Belgium





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            * Main Projects
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            - Technology Agency of the Czech Republic (2018 –2019)

            - Ministry of Industry and Trade of the Czech Republic (2017 –2021)
            - Innovative Technologies for the Identification and Optimization of the New Generation of
            Drugs (Charles University, UNCE 204025/2012, 2012–2016)

            - Innovation voucher (with BARD, s.r.o. 2015)





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            * Achievements
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            Publications in respected international journals: Plant Science, Biochimie, Journal of Pla

            Food Bioscience, FEBS Journal, Photosynthetica





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            * Are you interested in this expertise?
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            Please contact CPPT UK
            Web: www.cppt.cuni.cz/ [ URL "https://cppt.cuni.cz/"]
            Mail: transfer@cuni.cz

            Phone: +420 224 491 255





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            * Experts and their department
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            Assoc. Prof. RNDr. Helena Ryšlavá, CSc.

            Department of Biochemistry
            Web: http://web.natur.cuni.cz/~biochem/main/biochemie_rostlin/ [ URL "http://web.natur.cun
            main/biochemie_rostlin/"]