Laboratory of Electrophoretic Separation Methods ****************************************************************************************** * ****************************************************************************************** ****************************************************************************************** * Offer ****************************************************************************************** - Development and testing of new methods and instrumentation for microscale separation - Expertise in the area of microscale separation techniques - Cooperation with partners on development and construction of electrochemical detectors f separation techniques - Cooperation on development of electrophoretic strategies for rapid determination of vari molecules in clinical samples (usable in various pharmacological and physiological studies - Cooperation with technological companies and research groups in this field ****************************************************************************************** * Know-how & Technologies ****************************************************************************************** - New methodologies based on electromigration for determination of analytes in clinical an environmental matrices - Very short analysis time, very little sample volume is required - Development of instrumentation for electrochemical detection and sample introduction in separation techniques ****************************************************************************************** * Research Area & Excelence ****************************************************************************************** - Development of new electrophoretic strategies for rapid determination of amino acids, sa and other metabolites in various clinical samples that represents background for solution pharmacological and physiological studies - Construction and optimization of electrochemical detectors for microscale separation tec - Development of new preconcentration techniques of biological samples that are based on e principle ****************************************************************************************** * Key Research Equipment ****************************************************************************************** - Agilent 7100 Capillary Electrophoresis System and HP3D Capillary Electrophoresis System Technologies) equipped with diod-array detector, contactless conductivity detector, fluore ARGOS 250B (Flux Instruments) and mass spectrometry detector CE/MS Single Quad ES Superior (Agilent Technologies) - Microchip electrophoresis with a lab-build contactless detector - Fully equipped biochemical laboratory for preparation and processing of the samples ****************************************************************************************** * Achievements ****************************************************************************************** Technique of sampling low volume analytes (as low as 1 µl) is currently in the process of property protection. ****************************************************************************************** * Partnerships & Collaborations ****************************************************************************************** Department of Analytical Chemistry, Faculty of Science, Charles University in Prague | J. Institute of Physical Chemistry | Institute of Physics | Department of Sport Medicine, Thi of Medicine, Charles University in Prague | 2nd Internal Department of Third Faculty of Me Faculty Hospital Královské Vinohrady | Centre for Research on Diabetes, Metabolism and Nut University in Prague | Department of Pharmacology, Third Faculty of Medicine, Charles Univ ****************************************************************************************** * Important Publications ****************************************************************************************** - Tůma P., Opekar F., Jelínek I.: A Contactless Conductometric Detector with Easily Exchangeable Capillary for Capillary Electrophoresis, Electroanalysis 2001, 13, 989–992. h dx.doi.org/10.1002/1521-4109(200108)13:12<989::AID-ELAN989>3.0.CO;2-C, IF2001 1,702, 59 ti - Tůma P., Opekar F., Štulík K.: Contactless conductivity detector for capillary zone elec effect of the detection cell geometry on the detector performance, Electrophoresis 2002, 2 http://dx.doi.org/10.1002/1522-2683(200211)23:21<3718::AID-ELPS3718> 3.0.CO;2-U, IF2002 4, cited. - Tůma P., Samcová E., Andělová K.: Determination of free amino acids and related compound fluid by capillary electrophoresis with contactless conductivity detection, J. Chromatogr. 18. http://dx.doi.org/10.1016/j.jchromb.2005.12.020, IF2006 2,647, 52 times cited. - Tůma P., Samcová E., Opekar F., Jurka V. and Štulík K.: Determination of 1-methylhistidi 3-methylhistidine by capillary and chip electrophoresis with contactless conductivity dete Electrophoresis 2007, 28, 2174–2180. http://dx.doi.org/10.1002/ elps.200600697, IF2007 3,6 cited. - Tůma P., Samcová E., Duška F.: Determination of Ammonia, Creatinine and Inorganic Cation CE with Contactless Conductivity Detection, J. Sep. Sci. 2008, 31, 2260–2264. http:// dx.d jssc.200700655, IF2008 2,746, 25 times cited. - Tůma P., Málková K., Samcová E., Štulík K.: Rapid Monitoring of Arrays of Amino Acids in Using Capillary Electrophoresis with Contactless Conductivity Detection, J. Sep. Sci. 2010 http://dx.doi.org/10.1002/jssc.201000137, IF2010 2,631, 31 times cited. ****************************************************************************************** * Are you interested in this expertise? ****************************************************************************************** Please contact CPPT UK Web: www.cppt.cuni.cz/ [ URL "https://cppt.cuni.cz/"] Mail: transfer@cuni.cz Phone: +420 224 491 255 ****************************************************************************************** * Experts and their department ****************************************************************************************** Assoc. Prof. Ing. RNDr. Petr Tůma, Phd. Department of Hygiene Web: https://www.lf3.cuni.cz/3LFEN-325.html [ URL "https://www.lf3.cuni.cz/3LFEN-325.html"